Haemato‐immunological response of Labeo rohita (Hamilton) fingerlings fed leaf extracts and challenged by Aeromonas hydrophila
Haemato‐immunological response of Labeo rohita (Hamilton) fingerlings fed leaf extracts and challenged by Aeromonas hydrophila
复制标题
喂食叶提取物并受到嗜水气单胞菌攻击的 Labeo rohita（汉密尔顿）鱼种的血液免疫反应
DOI：
10.1111/are.12829
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
A. Ravindran
中科院分区：
文献类型：
--
作者：
F. J. Fawole;N. P. Sahu;A. K. Pal;A. Ravindran
通过35天的饲养试验，评价了饲喂番石榴叶乙醇提取物和芒果叶提取物后感染嗜水气单胞菌的Labeo Rohita鱼种的血液免疫学反应。将6种等氮(354.6-361.6 mg/kg−-1)纯净日粮按不同水平的叶提取物配制，即：对照组(不加任何提取物的基础饲料)、TG-5(5 mg/kg−1番石榴提取物)、TG-10(10 g/kg−1番石榴提取物)、TM-5(5 g/kg−1芒果提取物)、TM-10(10 g/kg−1芒果提取物)和TGM(5 g/kg−1番石榴提取物+5 g/kg−1芒果提取物)。在35天的喂养试验和7天的嗜水气单胞菌挑战之后，对血液学、免疫学、生化以及抗氧化酶活性进行了检测。在攻毒前和攻毒后条件下，与对照组相比，叶提取物组的血红蛋白、总白细胞和红细胞计数、呼吸爆发活性、溶菌酶、总蛋白、白蛋白和球蛋白含量均显著升高(P<0.05)。与对照组相比，治疗组超氧化物歧化酶和过氧化氢酶活性显著降低(P<0.05)。死亡率趋势表明，血液免疫反应显著增强的鱼群在受到嗜水气单胞菌攻击后死亡率最低。结果表明，红番石榴和红番石榴提取物在饲料中的−-1含量为5 mg/kg时，具有潜在的免疫刺激作用。但是，两种提取物在类似水平的混合并没有表现出任何协同作用，这需要在较低的包合水平上进行测试。
A 35 days feeding trial was conducted to assess the haemato-immunological response of Labeo rohita fingerlings fed ethanolic leaf extracts of Psidium guajava and Mangifera indica, and infected with Aeromonas hydrophila. Six iso-nitrogenous (354.6–361.6 g kg−1) purified diets were prepared with graded level of leaf extracts viz., control (basal feed without any extract); TG-5 (5 g kg−1 guava extract); TG-10 (10 g kg−1 guava extract); TM-5 (5 g kg−1 mango extract); TM-10 (10 g kg−1 mango extract); and TGM (5 g kg−1 guava extract +5 g kg−1 mango extract). Haematological, immunological, biochemical, along with antioxidant enzyme activities were examined after a 35 day-feeding trial and following a 7 day challenge with A. hydrophila. The haemoglobin, total leucocyte and erythrocyte counts, respiratory burst activity, lysozyme, total protein, albumin and globulin contents increased significantly (P < 0.05) in leaf extracts fed groups compared with the control in pre- and post-challenge conditions. A significant (P < 0.05) decrease was observed in SOD (superoxide dismutase) and catalase activities of the treatment groups compared with the higher value in control. The trends in mortality indicated that groups of fish showing significantly elevated haemato-immunological responses had the lowest mortality following challenge with A. hydrophila. The results showed that extracts of P. guajava and M. indica appear to be potential immunostimulant at an inclusion level of 5 g kg−1 in the diet of rohu. But, mixing of both the extract at similar level did not show any synergistic effect, which needs to be tested at its lower level of inclusion.