Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.
Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.
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DOI：
10.1093/dnares/3.5.321
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发表时间：
1996-10-31
期刊：
影响因子：
--
通讯作者：
Nomura, N
中科院分区：
文献类型：
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作者：
Nagase, T;Seki, N;Nomura, N
在这一系列测序人类cDNA克隆的项目中，我们新测定了80个克隆的序列，并预测了相应基因的编码序列，命名为KIAA 0201至KIAA 0280。在测序的克隆中，68个来自人未成熟骨髓细胞系KG-1，12个来自人脑。克隆的平均大小为5.3 kb，克隆中不同ORF的平均大小为2.8 kb，对应于约100 kDa的蛋白质。对公共数据库的计算机搜索表明，22个基因的序列与任何已报道的基因无关，而其余58个基因的序列与已知基因有一定的相似性。在25个基因中发现了与PROSITE基序数据库中匹配的蛋白质基序，在30个基因中鉴定了重要的跨膜结构域。在已知的基因，显着的相似性显示，在发育阶段，细胞凋亡和细胞间相互作用的调控中发挥关键作用的基因。考虑到序列相似性和蛋白质基序的搜索数据，至少有7个基因被认为与转录调控相关，6个基因与信号转导相关。当用不同的人体组织检查cDNA克隆的表达谱时，约一半的来自脑的克隆（11个中的5个）显示出显著的组织特异性，而来自KG-1的约80%的基因普遍表达。
In this series of projects of sequencing human cDNA clones which correspond to relatively long and nearly full-length transcripts, we newly determined the sequences of 80 clones, and predicted the coding sequences of the corresponding genes, named KIAA0201 to KIAA0280. Among the sequenced clones, 68 were obtained from human immature myeloid cell line KG-1 and 12 from human brain. The average size of the clones was 5.3 kb, and that of distinct ORFs in clones was 2.8 kb, corresponding to a protein of approximately 100 kDa. Computer search against the public databases indicated that the sequences of 22 genes were unrelated to any reported genes, while the remaining 58 genes carried sequences which show some similarities to known genes. Protein motifs that matched those in the PROSITE motif database were found in 25 genes and significant transmembrane domains were identified in 30 genes. Among the known genes to which significant similarity was shown, the genes that play key roles in regulation of developmental stages, apoptosis and cell-to-cell interaction were included. Taking into account of both the search data on sequence similarity and protein motifs, at least seven genes were considered to be related to transcriptional regulation and six genes to signal transduction. When the expression profiles of the cDNA clones were examined with different human tissues, about half of the clones from brain (5 of 11) showed significant tissue-specificity, while approximately 80% of the genes from KG-1 were expressed ubiquitously.