Estimating SIT-driven population reduction in the Mediterranean fruit fly, Ceratitis capitata, from sterile mating
Estimating SIT-driven population reduction in the Mediterranean fruit fly, Ceratitis capitata, from sterile mating
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DOI：
10.1017/s0007485313000692
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发表时间：
2014-04-01
影响因子：
1.9
通讯作者：
Urbaneja, A.
中科院分区：
文献类型：
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作者：
Juan-Blasco, M.;Sabater-Munoz, B.;Urbaneja, A.
区域性不育昆虫技术（SIT）计划假设目标种群的后代减少与释放的不育雄性的交配成功相关。然而，缺乏实时监测工具来证明这些方案的成功。在西班牙地中海沿岸的环境条件下进行了田间笼试验，以评估：（a）使用分子标记的不育维也纳-8（V8）Ceratitis capitata雄性的交配成功率和（B）它们降低C.结果表明，在野生雌、雄、V8雄6种释放比例（1：0：0、1：1：0、1：1：1、1：1：5、1：1：10和1：1：20）下，capitata种群的种群数量和种群密度均显著高于野生雌、雄、V8雄种群。建立了统计模型，以预测：（a）陷阱中捕获的雌性数量，（B）捕获雌性受精囊中的精子ID（无菌或无菌），以及（c）产生的存活后代，使用释放率和温度作为预测因子。捕获的雌性数量受相对湿度的影响。然而，它在模型中的影响力很低。与释放V8雄性的比率相比，1：0：0的雌性捕获率显著较高。受精囊中V8精子的比例随温度和释放的V8雄性数量而增加，但在1：1：10和1：1：20之间趋于平稳。在所有季节，除了冬季（没有后代），可行的后代增加与温度和比例最低的1：1：20。首次证实了用分子生物学方法检测到的V8精子比例与C.后代的后代被建立。所获得的模型应有助于提高效率的SIT程序对这种害虫。
Area-wide sterile insect technique (SIT) programs assume that offspring reduction of the target population correlates with the mating success of the sterile males released. However, there is a lack of monitoring tools to prove the success of these programs in real-time. Field-cage tests were conducted under the environmental conditions of the Mediterranean coast of Spain to estimate:(a) the mating success of sterile Vienna-8 (V8) Ceratitis capitata males using molecular markers and (b) their efficacy to reduce C. capitata populations under six release ratios of wild females to wild males to V8 males (1:0:0, 1:1:0, 1:1:1, 1:1:5, 1:1:10, and 1:1:20). Statistical models were developed to predict:(a) the number of females captured in traps, (b) sperm ID (sterile or not) in spermathecae of the trapped females, and (c) the viable offspring produced, using release ratio and temperature as predictors. The number of females captured was affected by relative humidity. However, its influence in the model was low. Female captures were significantly higher in ratios 1:0:0 compared to ratios where V8 males were released. The proportion of V8 sperm in spermathecae increased with temperature and with the number of V8 males released, but leveled off between ratios 1:1:10 and 1:1:20. In all seasons, except winter (no offspring), viable offspring increased with temperature and was lowest for ratio 1:1:20. For the first time, a strong negative relationship between proportion of V8 sperm detected by molecular tools and C. capitata offspring was established. The models obtained should contribute to enhance the efficacy of SIT programs against this pest.