Compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor
Compressed mints and chewing gum containing magnolia bark extract are effective against bacteria responsible for oral malodor
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DOI：
10.1021/jf072122h
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发表时间：
2007-11-14
影响因子：
6.1
通讯作者：
Tian, Minmin
中科院分区：
文献类型：
--
作者：
Greenberg, Michael;Urnezis, Philip;Tian, Minmin
香精和天然植物提取物经常用于口香糖和压缩薄荷糖中，用于口气清新和缓解口腔恶臭。口腔恶臭是来自食物或唾液的蛋白质物质的细菌腐败的结果。采用最小抑菌浓度（MIC）法对厚朴提取物（MBE）及其主要成分厚朴酚和和厚朴酚进行了体外抑菌试验。MBE薄荷的抑制作用进一步评估了通过一个时间分析研究。此外，在午餐后对9名健康志愿者进行了体内研究。在受试者食用薄荷糖和口香糖之前和之后，在有和没有MBE的情况下采集唾液样本。包括李施德林漱口水作为阳性对照。测试结果显示，MBE及其两种主要成分对引起口臭的细菌和与龋齿形成有关的变形链球菌有很强的杀菌作用。厚朴酚、和厚朴酚和MBE对牙龈卟啉单胞菌、具核梭杆菌和S.变形杆菌的范围为8 - 31 μ g/mL。杀灭时间测定结果表明，含有0.2%MBE的薄荷糖在治疗5分钟内减少了99.9%以上的三种口腔细菌。体内研究表明，含有MBE的薄荷糖在食用后30分钟和60分钟分别使总唾液细菌减少61.6%和33.8%。相比之下，无味薄荷减少了3.6%的总唾液细菌在30分钟和增加了47.9%的总细菌在60分钟。MBE含有口香糖减少了43.0%的总唾液细菌在40分钟，而安慰剂口香糖减少了18.0%的总唾液细菌。总之，MBE表现出显着的抗菌活性，对有机体负责口腔恶臭，并可以纳入压缩薄荷糖和口香糖改善口气清新的好处。
Flavors and natural botanic extracts are often used in chewing gum and compressed mints for breath freshening and relief of oral malodor. The oral malodor is a result of bacterial putrification of proteinaceous materials from food or saliva. In this study, magnolia bark extract (MBE) and its two main components, magnolol and honokiol, were evaluated by the minimum inhibition concentration (MIC) test. The inhibitory effect of MBE mint was further evaluated by a kill-time assay study. In addition, an in vivo study was performed on nine healthy volunteers postlunch. Saliva samples were taken before and after subjects consumed mints and gum, with and without MBE. Listerine mouthwash was included as a positive control. The testing results indicated that MBE and its two main constituents demonstrated a strong germ-kill effect against bacteria responsible for halitosis and also Streptococcus mutans, bacteria involved in dental caries formation. The MIC of magnolol, honokiol, and MBE on Porphyromonas gingivalis, Fusobacterium nucleatum, and S. mutans ranged from 8 to 31 mu g/mL. Kill-time assay results indicated that mints containing 0.2% MBE reduced more than 99.9% of three oral bacteria within 5 min of treatment. The in vivo study demonstrated that MBE containing mints reduced total salivary bacteria by 61.6% at 30 min and 33.8% at 60 min postconsumption. In comparison, the flavorless mint reduced total salivary bacteria by 3.6% at 30 min and increased total bacteria by 47.9% at 60 min. The MBE containing chewing gum reduced total salivary bacteria by 43.0% at 40 min, while placebo gum reduced total salivary bacteria by 18.0%. In conclusion, MBE demonstrated a significant antibacterial activity against organisms responsible for oral malodor and can be incorporated in compressed mints and chewing gum for-improved breath-freshening benefits.