Genome-wide association analysis reveals loci associated with resistance against Piscirickettsia salmonis in two Atlantic salmon (Salmo salar L.) chromosomes.
Genome-wide association analysis reveals loci associated with resistance against Piscirickettsia salmonis in two Atlantic salmon (Salmo salar L.) chromosomes.
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DOI：
10.1186/s12864-015-2038-7
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发表时间：
2015-10-24
期刊：
影响因子：
4.4
通讯作者：
Yáñez JM
中科院分区：
文献类型：
--
作者：
Correa K;Lhorente JP;López ME;Bassini L;Naswa S;Deeb N;Di Genova A;Maass A;Davidson WS;Yáñez JM
鲑鱼立克次氏体病（Pisciricketssia salmonis）是鲑鱼立克次氏体综合征（SRS）的病原体，其影响鲑鱼物种并造成严重的经济损失。抗病性的选择性育种是控制养殖大西洋鲑鱼SRS的一种方法。在决定最合适的方法来增强大西洋鲑鱼对鲑鱼疫症抗性的人工选择之前，需要关于抗性性状的结构的知识。这项研究的目的是解剖大西洋鲑鱼对这种病原体的抗性的遗传变异。通过腹膜内注射的方式，对2，601只大西洋鲑鱼幼鱼进行实验性攻击以对抗鲑鱼附红细胞瘤。这些小鲑鱼是来自智利育种群体的40头公鹿和118头母鹿的后代。每天记录死亡率，实验在接种后第40天结束。使用50 K Affyphase ® Axiom® myDesignTM单核苷酸多态性（SNP）基因分型阵列对鱼进行基因分型。对来自受攻击鱼的数据进行全基因组关联分析。线性回归和逻辑回归模型进行了测试。全基因组关联分析表明，抗鲑性是一个中度多基因性状。在Ssa 01和Ssa 17染色体上发现了5个与性状显著相关的SNPs。每个标记解释的表型方差的比例很小，范围从0.007到0.045。候选基因包括白细胞介素受体和岩藻糖基转移酶已被发现与这些遗传标记物的物理联系，并可能在针对该病原体的差异免疫应答中发挥重要作用。由于每个显着的标记解释的方差很小，我们得出结论，这种病原体的遗传抗性可以更有效地提高与实施的遗传评估，将基因型信息从一个密集的SNP阵列。本文的在线版本（doi：10.1186/s12864-015-2038-7）包含补充材料，可供授权用户使用。
Pisciricketssia salmonis is the causal agent of Salmon Rickettsial Syndrome (SRS), which affects salmon species and causes severe economic losses. Selective breeding for disease resistance represents one approach for controlling SRS in farmed Atlantic salmon. Knowledge concerning the architecture of the resistance trait is needed before deciding on the most appropriate approach to enhance artificial selection for P. salmonis resistance in Atlantic salmon. The purpose of the study was to dissect the genetic variation in the resistance to this pathogen in Atlantic salmon. 2,601 Atlantic salmon smolts were experimentally challenged against P. salmonis by means of intra-peritoneal injection. These smolts were the progeny of 40 sires and 118 dams from a Chilean breeding population. Mortalities were recorded daily and the experiment ended at day 40 post-inoculation. Fish were genotyped using a 50K Affymetrix® Axiom® myDesignTM Single Nucleotide Polymorphism (SNP) Genotyping Array. A Genome Wide Association Analysis was performed on data from the challenged fish. Linear regression and logistic regression models were tested. Genome Wide Association Analysis indicated that resistance to P. salmonis is a moderately polygenic trait. There were five SNPs in chromosomes Ssa01 and Ssa17 significantly associated with the traits analysed. The proportion of the phenotypic variance explained by each marker is small, ranging from 0.007 to 0.045. Candidate genes including interleukin receptors and fucosyltransferase have been found to be physically linked with these genetic markers and may play an important role in the differential immune response against this pathogen. Due to the small amount of variance explained by each significant marker we conclude that genetic resistance to this pathogen can be more efficiently improved with the implementation of genetic evaluations incorporating genotype information from a dense SNP array. The online version of this article (doi:10.1186/s12864-015-2038-7) contains supplementary material, which is available to authorized users.