Measurement of Thermoregulatory Behavior in Drosophila melanogaster
Measurement of Thermoregulatory Behavior in Drosophila melanogaster
复制标题
果蝇体温调节行为的测量
DOI：
10.1007/978-1-0716-2321-3_6
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Umeda Masato
中科院分区：
文献类型：
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作者：
Suito Takuto;Nagao Kohjiro;Kai Masahiro;Juni Naoto;Sokabe Takaaki;Umeda Masato
体温调节是一种基本的生理功能，在吸热和变温动物中维持体内平衡。果蝇（Drosophila melanogaster）作为模式生物，对行为遗传学的研究做出了显著的贡献。利用果蝇进行的行为分析揭示了感觉神经元、传感分子和其他因素，包括与体温调节密切相关的能量代谢。在这一章中，我们描述了分析果蝇体温调节行为所需的设备和程序。我们还提供了选择策略来分析果蝇的行为温度调节的指导方针。此外，我们提供了具有代表性的结果与这些协议，并表明，可以通过改变膜磷脂的不饱和度诱导在优选的温度的变化。
Thermoregulation is a fundamental physiological function that maintains homeostasis in both endothermic and ectothermic animals. As a model organism, the fruit fly (Drosophila melanogaster) has markedly contributed to the study of behavioral genetics. Behavioral analyses usingDrosophilahave revealed the sensory neurons, sensor molecules, and other factors including energy metabolism that are closely linked to thermoregulation. In this chapter, we describe the equipment and procedures required for analyzing thermoregulatory behavior inDrosophila. We also provide guidelines for the selection of strategies to analyze behavioral thermoregulation inDrosophila. In addition, we provide representative results obtained with these protocols and show that changes in preferred temperature can be induced by altering the unsaturation of membrane phospholipids.