Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium
Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium
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DOI：
10.1021/nl052326h
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发表时间：
2006-04-01
期刊：
影响因子：
10.8
通讯作者：
Fiévet, F
中科院分区：
文献类型：
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作者：
Brayner, R;Ferrari-Iliou, R;Fiévet, F
我们报道了纳米氧化锌对大肠杆菌的杀菌作用和细胞内化作用的初步研究。在二甘醇(DEG)介质中，通过强制水解离子锌盐合成了纳米氧化锌。通过添加三正辛基氧膦、十二烷基硫酸钠、聚氧乙烯硬脂醚和牛血清白蛋白等小分子和大分子来控制颗粒的大小和形状。用透射电子显微镜和X射线衍射仪对颗粒的结构、大小和形貌进行了表征。在Luria-Bertani培养基、固体琼脂平板和含有不同浓度的小分子和大分子的液体体系中进行了杀菌试验，并与纳米氧化锌进行了比较。利用细菌切片的透射电子显微镜分析，研究了氧化锌材料的杀菌作用。结果证实，DEG与氧化锌接触后对大肠杆菌细胞有损伤作用，表现为革兰氏阴性三重膜结构紊乱。这种行为导致膜通透性的增加，导致纳米氧化锌在细菌膜上的积累，并使这些纳米颗粒在细胞中内化。
We report here preliminary studies of blocidal effects and cellular internalization of ZnO nanoparticles on Escherichia coli bacteria. ZnO nanoparticles were synthesized in di(ethylene glycol) (DEG) medium by forced hydrolysis of ionic Zn2+ salts. particle size and shape were controlled by addition of small molecules and macromolecules such as tri-n-octylphosphine oxide, sodium dodecyl sulfate, polyoxyethylene stearyl ether, and bovine serum albumin. Transmission electron microscopy (TEM) and X-ray diffraction analyses were used to characterize particle structure, size, and morphology. Bactericidal tests were performed in Luria-Bertani medium on solid agar plates and in liquid systems with different concentrations of small and macromolecules and also with ZnO nanoparticles. TEM analyses of bacteria thin sections were used to study biocidal action of ZnO materials. The results confirmed that E. coli cells after contact with DEG and ZnO were damaged showing a Gram-negative triple membrane disorganization. This behavior causes the increase of membrane permeability leading to accumulation of ZnO nanoparticles in the bacterial membrane and also cellular internalization of these nanoparticles.