Myxococcus xanthus sasN encodes a regulator that prevents developmental gene expression during growth.
Myxococcus xanthus sasN encodes a regulator that prevents developmental gene expression during growth.
复制标题
黄粘球菌 sasN 编码一种调节因子，可防止生长过程中发育基因的表达。
DOI：
10.1128/jb.180.23.6215-6223.1998
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发表时间：
1998
影响因子：
3.2
通讯作者：
Kaplan,HB
中科院分区：
文献类型：
--
作者：
Xu,D;Yang,C;Kaplan,HB
Myxococcus xanthusmulticellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the4521developmental reporter gene. These mutants express4521at high levels during growth and development in anasgBbackground, which is defective in generation of the cell density signal, A signal. A 1.3-kb region of thesasBlocus cloned from the wild-type chromosome restored the SasB+phenotype to the five mutants. DNA sequence analysis of the 1.3-kb region predicted an open reading frame, designated SasN. The N terminus of SasN appears to contain a strongly hydrophobic region and a leucine zipper motif. SasN showed no significant sequence similarities to known proteins. A strain containing a newly constructedsasN-null mutation and Ω4521 Tn5lacin an otherwise wild-type background expressed4521at a high level during growth and development. A similarsasN-null mutant formed abnormal fruiting bodies and sporulated at about 10% the level of wild type. These data indicate that the wild-typesasNgene product is necessary for normalM. xanthusfruiting body development and functions as a critical regulator that prevents4521expression during growth.
