A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes.
A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes.
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DOI：
10.1083/jcb.200803150
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发表时间：
2008-06-16
影响因子：
7.8
通讯作者：
Pfister, K. Kevin
中科院分区：
文献类型：
--
作者：
Ha, Junghoon;Lo, Kevin W. -H.;Myers, Kenneth R.;Carr, Tiffany M.;Humsi, Michael K.;Rasoul, Bareza A.;Segal, Rosalind A.;Pfister, K. Kevin
Cytoplasmic dynein is the multisubunit motor protein for retrograde movement of diverse cargoes to microtubule minus ends. Here, we investigate the function of dynein variants, defined by different intermediate chain (IC) isoforms, by expressing fluorescent ICs in neuronal cells. Green fluorescent protein (GFP)–IC incorporates into functional dynein complexes that copurify with membranous organelles. In living PC12 cell neurites, GFP–dynein puncta travel in both the anterograde and retrograde directions. In cultured hippocampal neurons, neurotrophin receptor tyrosine kinase B (TrkB) signaling endosomes are transported by cytoplasmic dynein containing the neuron-specific IC-1B isoform and not by dynein containing the ubiquitous IC-2C isoform. Similarly, organelles containing TrkB isolated from brain by immunoaffinity purification also contain dynein with IC-1 but not IC-2 isoforms. These data demonstrate that the IC isoforms define dynein populations that are selectively recruited to transport distinct cargoes.
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