Croceibacter atlanticus gen. nov., sp nov., a novel marine bacterium in the family Flavobacteriaceae
Croceibacter atlanticus gen. nov., sp nov., a novel marine bacterium in the family Flavobacteriaceae
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DOI：
10.1078/072320203322337344
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发表时间：
2003-03-01
影响因子：
3.4
通讯作者：
Giovannoni, SJ
中科院分区：
文献类型：
--
作者：
Cho, JC;Giovannoni, SJ
采用高通量培养方法从大西洋马尾藻海西部的百慕大大西洋时间序列站分离到一株明亮的、淡紫色的海洋细菌HTCC 2559（T），并对其进行了多相鉴定。表型数据和系统发育分析表明，该菌株是黄杆菌科的成员。该菌株是革兰氏阴性，非能动，化能异养，严格需氧，NaCl需要，杆状细胞，含有类胡萝卜素色素，但不含flexirubin。几种大分子（明胶，DNA，淀粉，酪蛋白和弹性蛋白）被降解，碳水化合物，糖醇，有机酸和氨基酸被用作唯一的碳源。主要脂肪酸为支链酸或羟基酸，3-OH i 17：0、i 15：0、i 15：1和i 17：1 ω 9 c含量丰富。DNA G+C含量为34.8mol%。基于16 S rRNA基因序列的系统发育分析表明，该菌株形成了一个非常独特的谱系，与黄杆菌科中的几个海水环境克隆密切相关。因此，从多相研究中提出菌株HTCC 2559（T）（=ATCC BAA-628（T）= KCTC 12090（T））属于一个新的属和种，命名为Croceibacter atlanticus gen. nov.，sp. Nov.
A bright, saffron-colored marine bacterium HTCC2559(T) was isolated from the Bermuda Atlantic Time Series station in the western Sargasso Sea, Atlantic Ocean by high throughput Culturing methods and characterized by polyphasic approaches. Phenotypic data and phylogenetic analyses showed that the strain is a member of the family Flavobacteriaceae. The strain was Gram-negative, non-motile, chemoheterotrophic, strictly aerobic, NaCl-requiring, rod-shaped cells that contain carotenoid pigments but not flexirubin. Several kinds of macromolecules (gelatin, DNA, starch, casein, and elastin) were degraded and carbohydrates, sugar alcohols, organic acids, and amino acids were utilized as sole carbon Sources. The dominant fatty acids were branched or hydroxy acids, and 3-OH i17:0, i15:0, i15: 1, and i17:1 omega9c were abundant. The DNA G+C content of the strain is 34.8 mol%. Phylogenetic analyses using three treeing algorithms based on 16S rRNA gene sequences revealed that the strain formed a very distinct lineage that is allied closely with several seawater environmental clones in the family Flavobacteriaceae. Therefore, it is proposed from the polyphasic studies that strain HTCC2559(T) (=ATCC BAA-628(T) = KCTC 12090(T)) belongs to a new genus and species named Croceibacter atlanticus gen. nov., sp. nov.