Protocadherin PAPC is expressed in the CNC and can compensate for the loss of PCNS
Protocadherin PAPC is expressed in the CNC and can compensate for the loss of PCNS
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DOI：
10.1002/dvg.22736
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发表时间：
2014-02
期刊：
影响因子：
1.5
通讯作者：
Martina Schneider;Chaolie Huang;Sarah F. S. Becker;D. Gradl;D. Wedlich
中科院分区：
文献类型：
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作者：
Martina Schneider;Chaolie Huang;Sarah F. S. Becker;D. Gradl;D. Wedlich
原钙粘蛋白是跨膜糖蛋白钙粘蛋白超家族中最大的亚类。与经典的I型钙粘蛋白相反，原钙粘蛋白通常仅表现出中等的粘附活性。在胚胎发生期间，它们参与细胞信号传导并调节多种形态发生过程，包括原肠胚形成期间的形态发生运动和神经嵴迁移。近轴原钙粘蛋白（PAPC）和轴原钙粘蛋白（AXPC）是非洲爪蟾和斑马鱼原肠胚形成过程中不可缺少的两种原钙粘蛋白。相反，最接近的相关PCNS是神经嵴和体节形成所必需的。在这里，我们表明，颅神经嵴（CNC）细胞除了PCNS表达PAPC，但不AXPC。PAPC的过表达导致与PCNS敲低相当的迁移缺陷。此外，重建实验表明，PAPC能够取代CNC细胞中的PCNS，表明两种原钙粘蛋白都可以调节CNC迁移。创世纪52：120-126。© 2013 Wiley Periodicals，Inc.
Protocadherins represent the biggest subgroup within the cadherin superfamily of transmembrane glycoproteins. In contrast to classical type I cadherins, protocadherins in general exhibit only moderate adhesive activity. During embryogenesis, they are involved in cell signaling and regulate diverse morphogenetic processes, including morphogenetic movements during gastrulation and neural crest migration. The two protocadherins paraxial protocadherin (PAPC) and axial protocadherin (AXPC) are indispensable for proper gastrulation movements in Xenopus and zebrafish. The closest relative PCNS instead, is required for neural crest and somite formation. Here, we show that cranial neural crest (CNC) cells in addition to PCNS express PAPC, but not AXPC. Overexpression of PAPC resulted in comparable migration defects as knockdown of PCNS. Moreover, reconstitution experiments revealed that PAPC is able to replace PCNS in CNC cells, indicating that both protocadherins can regulate CNC migration. genesis 52:120–126. © 2013 Wiley Periodicals, Inc.