Altered oral viral ecology in association with periodontal disease.
Altered oral viral ecology in association with periodontal disease.
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DOI：
10.1128/mbio.01133-14
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发表时间：
2014-05-20
期刊：
影响因子：
6.4
通讯作者：
Pride DT
中科院分区：
文献类型：
--
作者：
Ly M;Abeles SR;Boehm TK;Robles-Sikisaka R;Naidu M;Santiago-Rodriguez T;Pride DT
人类口腔是一个庞大而多样的病毒群落的家园，这些病毒尚未在牙周病患者中得到表征。我们从牙周健康或患有轻度或严重牙周疾病的人群中招募并采样唾液和口腔生物膜，以辨别是否存在反映其口腔健康状况的病毒群落差异。我们发现，每个受试者的唾液和龈下及龈上生物膜中存在大量由噬菌体组成的病毒群落。虽然有同源病毒常见于不同的科目和口腔粘膜的网站，对于大多数科目，病毒组的组成显着相关的口腔网站，他们来自。当比较龈下和龈上生物膜与牙周炎唾液时，发现病毒组成分之间的最大区别。龈下和龈上生物膜病毒的病毒组组成差异与口腔健康状况显著相关，但与唾液病毒无关。在病毒组成分中发现的差异是龈下生物膜中肌病毒的显著扩增，表明牙周病有利于裂解性噬菌体。我们还通过使用16S rRNA的V3高变片段来表征每个受试者在每个细菌群落中的细菌群落，并且没有识别出与病毒群落中发现的相似的口腔健康和疾病之间的区别。牙周病患者口腔生物膜中病毒生态的显著改变表明，与牙周健康的人相比，病毒可能是口腔健康状况的有用指标。关于病毒作为人类微生物组成员的作用或成分知之甚少。我们调查了一组相对牙周健康的受试者或严重牙周炎患者的口腔病毒群落组成，以确定健康状况是否与病毒差异相关。我们发现大多数病毒都是细菌的捕食者。根据口腔健康状况，居住在牙菌斑中的病毒存在显著差异，而唾液中的病毒则没有差异。牙周炎中的牙菌斑病毒被预测比健康口腔中的牙菌斑病毒更有可能杀死它们的细菌宿主。由于牙周炎等口腔疾病已被证明改变了细菌群落，我们认为病毒及其作为生态系统多样性驱动因素的作用是人类口腔微生物组健康和疾病状态的重要贡献者。
The human oral cavity is home to a large and diverse community of viruses that have yet to be characterized in patients with periodontal disease. We recruited and sampled saliva and oral biofilm from a cohort of humans either periodontally healthy or with mild or significant periodontal disease to discern whether there are differences in viral communities that reflect their oral health status. We found communities of viruses inhabiting saliva and the subgingival and supragingival biofilms of each subject that were composed largely of bacteriophage. While there were homologous viruses common to different subjects and biogeographic sites, for most of the subjects, virome compositions were significantly associated with the oral sites from which they were derived. The largest distinctions between virome compositions were found when comparing the subgingival and supragingival biofilms to those of planktonic saliva. Differences in virome composition were significantly associated with oral health status for both subgingival and supragingival biofilm viruses but not for salivary viruses. Among the differences identified in virome compositions was a significant expansion of myoviruses in subgingival biofilm, suggesting that periodontal disease favors lytic phage. We also characterized the bacterial communities in each subject at each biogeographic site by using the V3 hypervariable segment of the 16S rRNA and did not identify distinctions between oral health and disease similar to those found in viral communities. The significantly altered ecology of viruses of oral biofilm in subjects with periodontal disease compared to that of relatively periodontally healthy ones suggests that viruses may serve as useful indicators of oral health status. Little is known about the role or the constituents of viruses as members of the human microbiome. We investigated the composition of human oral viral communities in a group of relatively periodontally healthy subjects or significant periodontitis to determine whether health status may be associated with differences in viruses. We found that most of the viruses present were predators of bacteria. The viruses inhabiting dental plaque were significantly different on the basis of oral health status, while those present in saliva were not. Dental plaque viruses in periodontitis were predicted to be significantly more likely to kill their bacterial hosts than those found in healthy mouths. Because oral diseases such as periodontitis have been shown to have altered bacterial communities, we believe that viruses and their role as drivers of ecosystem diversity are important contributors to the human oral microbiome in health and disease states.