The presence of free D-alanine, D-proline and D-serine in mice.
The presence of free D-alanine, D-proline and D-serine in mice.
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小鼠体内存在游离 D-丙氨酸、D-脯氨酸和 D-丝氨酸。
DOI：
10.1016/0304-4165(92)90055-y
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Toyoaki Akino
中科院分区：
文献类型：
--
作者：
Yoko Nagata;Kazuhiko Yamamoto;Ta;R. Konno;Yosihiro Yasumura;Toyoaki Akino
用缺乏d-氨基酸氧化酶的突变小鼠品系证明了哺乳动物组织中游离丙氨酸、d-脯氨酸和d-丝氨酸的存在。本实验以1-氟-2，4-二硝基苯基-5-L-丙氨酸酰胺（FDAA）为衍生试剂，将肾脏和血清中的游离氨基酸衍生为非对映异构体，用二维薄层色谱（TLC）分离，用反相高效液相色谱（HPLC）分析d-和l-异构体，根据HPLC峰面积计算丙氨酸、脯氨酸和丝氨酸的d/l比值。
The presence of freed-alanine,d-proline andd-serine was demonstrated in mammalian tissues, using a mutant mouse strain lackingd-amino acid oxidase. In the experiment, free amino acids from the kidney and serum were derivatized with 1-fluoro-2,4-dinitrophenyl-5-l-alanine amide (FDAA) to diastereomers, separated by two-dimensional thin-layer chromatography (TLC), and analysed by reversed-phase high-performance liquid chromatography (HPLC) for the resolution ofd- andl-isomers,d/lratios of alanine, proline and serine were obtained based on the peak areas of HPLC.