EXCHANGE AREA AND SURFACE-PROPERTIES OF THE MICROVASCULATURE OF THE RABBIT SUBMANDIBULAR-GLAND FOLLOWING DUCT LIGATION
EXCHANGE AREA AND SURFACE-PROPERTIES OF THE MICROVASCULATURE OF THE RABBIT SUBMANDIBULAR-GLAND FOLLOWING DUCT LIGATION
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DOI：
10.1113/jphysiol.1984.sp015387
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发表时间：
1984-01-01
影响因子：
5.5
通讯作者：
SMAJE, LH
中科院分区：
文献类型：
--
作者：
CLOUGH, G;SMAJE, LH
测量下颌下腺微血管的交换面积，以允许根据先前的渗透性-表面积（PS）乘积测量值计算微血管对亲水性溶质的渗透性（P）值。导管结扎2周的腺体和对侧对照腺体在用含有阳离子化铁蛋白的溶液灌注后用改良的Karnovsky固定剂灌注固定，并用透射电子显微镜检查。体视学技术被用来估计表面积的交换血管的随机薄切片从4个控制和4导管结扎的腺体。对照腺体的平均交换表面积为512 cm 2 g-1，导管结扎腺体为336 cm 2 g-1。计算出对照腺体的窗孔密度为交换表面的0.57%，导管结扎组织的窗孔密度为交换表面的0.30%。阳离子化的铁蛋白分子似乎与微血管内皮的腔表面结合，包括在对照和结扎的腺体中约25 nm深度的窗孔表面。下颌下腺有孔内皮的交换表面积与心肌相当，但对小溶质的渗透性（P）约为心肌的10倍。以下结扎的唾液腺导管溶质渗透性福尔斯和解释，根据减少的表面积和小溶质的渗透性-流量关系的性质提供。
The exchange area of the submandibular salivary gland microvasculature was measured to allow the value of microvascular permeability (P) to hydrophilic solutes to be calculated from previous measurements of permeability-surface area (PS) products. Glands whose ducts had been ligated for 2 wk and the contralateral control glands were perfusion-fixed with a modified Karnovsky''s fixative after perfusion with a solution containing cationized ferritin, and examined with transmission electron microscopy. Stereological techniques were used to estimate the surface area of the exchange vessels on random thin sections from 4 control and 4 duct-ligated glands. The mean exchange surface area in control glands was 512 cm2 g-1 and 336 cm2 g-1 in duct-ligated glands. The fenestral density was calculated to be 0.57% of the exchange surface in control glands and 0.30% in duct-ligated tissue. Molecules of cationized ferritin appeared bound to the luminal surface of the microvascular endothelium, including the surface ot the fenestrae to a depth of about 25 nm in both control and ligated glands. The exchange surface area of the fenestrated endothelium of the submandibular salivary gland is comparable to that in cardiac muscle but the permeability (P) to small solutes is about 10 times greater. Following ligation of the salivary gland duct solute permeability falls and an explanation of this, based on the reduced surface area and the nature of the permeability-flow relationship for small solutes is offered.