Characteristic Localization of gpl30 (the Signal‐transducing Receptor Component Used in Common for IL‐6/IL‐11/CNTF/LIF/OSM) in the Rat Brain
Characteristic Localization of gpl30 (the Signal‐transducing Receptor Component Used in Common for IL‐6/IL‐11/CNTF/LIF/OSM) in the Rat Brain
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gpl30（IL-6/IL-11/CNTF/LIF/OSM 常用的信号转导受体成分）在大鼠大脑中的特征定位
DOI：
10.1111/j.1460-9568.1996.tb01307.x
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发表时间：
1996
影响因子：
3.4
通讯作者：
H. Kiyama
中科院分区：
文献类型：
--
作者：
D. Watanabe;R. Yoshimura;M. Khalil;K. Yoshida;T. Kishimoto;T. Taga;H. Kiyama
通过使用gp 130特异性抗体的免疫组织化学证实了gp 130在大鼠脑中的定位，gp 130是白细胞介素（IL）-6、IL-11、睫状神经营养因子（CNTF）、LIF和OSM共同使用的信号转导受体组分。在神经胶质细胞和神经元细胞中均观察到gp 130免疫反应。观察到两种不同的神经元染色模式。第一种显示神经胶质细胞染色，主要在端脑结构中观察到，包括海马、大脑皮质和尾壳核。第二种模式是在神经元胞体的细胞质膜上观察到的，主要见于下脑干，网状结构的大神经元，以及脊髓和颅运动神经元。电子显微镜分析显示，两种类型的gpl 30免疫反应性主要与细胞质膜相关，并且不精确地定位在突触位点。此外，在少突胶质细胞和室管膜下区也观察到gp 130免疫反应性。这些广泛但特征性的gp 130免疫反应性模式与IL-6受体和CNTF α链的免疫反应性模式重叠，表明细胞因子和生长因子（如IL-6和CNTF）通过gp 130在脑的某些特定区域中发挥作用。
The localization of gp130, the signal transducing receptor component used in common for interleukin (IL)‐6, IL‐11, ciliary neurotrophic factor (CNTF), LIF and OSM, in the rat brain was demonstrated by immunohistochemistry using an antibody specific to gpl30. The gp130 immunoreactivity was observed in both glial and neuronal cells. Two distinct neuronal staining patterns were observed. The first showed neuropil staining, observed mainly in telencephalic structures including the hippocampus, cerebral cortex and caudate‐putamen. The second pattern was observed on the cytoplasmic membrane of neuronal somata and was found primarily in the lower brainstem, in the large neurons of the reticular formation, and in spinal and cranial motor neurons. Electron‐microscopic analysis revealed that both types of gpl30 immunoreactivity were primarily associated with the cytoplasmic membrane and were not localized exactly at synaptic sites. Further, gpl30 immunoreactivity was also observed in the oligodendrocytes and subependymal zone. These widespread but characteristic patterns of gp130 immunoreactivity overlap well with those of IL‐6 receptor and CNTF alpha chains, suggesting a role of cytokines and growth factors such as IL‐6 and CNTF via gp130 in certain specific regions of the brain.
影响因子：
4.4
作者：
Sunhee C. Lee;Wei Liu;D. Dickson;C. Brosnan;J. Berman
通讯作者：
Sunhee C. Lee;Wei Liu;D. Dickson;C. Brosnan;J. Berman
影响因子：
4.4
作者：
J. G. Norris;L. Tang;S. M. Sparacio;E. Benveniste
通讯作者：
J. G. Norris;L. Tang;S. M. Sparacio;E. Benveniste