Variation in permissiveness for broad-host-range plasmids among genetically indistinguishable isolates of Dickeya sp from a small field plot
Variation in permissiveness for broad-host-range plasmids among genetically indistinguishable isolates of Dickeya sp from a small field plot
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DOI：
10.1111/j.1574-6941.2010.00880.x
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发表时间：
2010-07-01
影响因子：
4.2
通讯作者：
Smalla, Kornelia
中科院分区：
文献类型：
--
作者：
Heuer, Holger;Ebers, Jonas;Smalla, Kornelia
植物病原种群需要遗传灵活性来适应不断改进的植物防御。由广寄主范围质粒转移的基因库为种群提供遗传变异。然而，一个群体必须平衡这种好处与获得有害外来DNA的风险。这可以通过调节对宽宿主范围质粒具有高或低容许性的个体的比例来实现。我们调查了质粒摄取是否在400平方米的田间地块的Dickeya sp.遗传上难以区分的菌株之间变化。从大肠杆菌到Dickeya的广泛宿主范围IncP-1质粒的转移频率在分离株之间存在显着差异。质粒pTH 10和pB 10的不同α-和β-亚组的IncP-1，分别的转移频率，相关性良好。这些质粒的数量级不同的许可的菌株是不可区分的其他表型性状分析，基因组指纹或hrpN基因序列。这些菌株被分离在附近和不同的地块的领域，表明一个合理的快速遗传机制之间的切换低和高的许可。
Phytopathogenic populations need genetic flexibility to adapt to continually improving plant defences. The gene pool transferred by broad-host-range plasmids provides genetic variation for the population. However, a population has to balance this benefit with the risk of acquiring deleterious foreign DNA. This could be achieved by modulating the ratio of individuals with high or low permissiveness to broad-host-range plasmids. We investigated whether plasmid uptake varied among genetically indistinguishable isolates of Dickeya sp. from a 400 m2 field plot. The transfer frequencies of broad-host-range IncP-1 plasmids from Escherichia coli to Dickeya differed significantly among isolates. The transfer frequencies for plasmids pTH10 and pB10 of the divergent alpha- and beta-subgroups of IncP-1, respectively, correlated well. Strains that differed in permissiveness for these plasmids by orders of magnitude were not distinguishable by other phenotypic traits analysed, by genomic fingerprints or hrpN gene sequences. Such strains were isolated in close vicinity and from different plots of the field, indicating a reasonably fast genetic mechanism of switching between low and high permissiveness.