Environmental fine particulate matter (PM 2.5) activates the RAW 264.7 macrophage cell line even at very low concentrations as revealed by 1H NMR
Environmental fine particulate matter (PM 2.5) activates the RAW 264.7 macrophage cell line even at very low concentrations as revealed by 1H NMR
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DOI：
10.1021/tx034118f
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发表时间：
2004-01-01
影响因子：
4.1
通讯作者：
Indovina, PL
中科院分区：
文献类型：
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作者：
Santini, MT;Rainaldi, G;Indovina, PL
由于空气中颗粒物（PM）的吸入与人类呼吸道和心血管疾病之间存在关联，因此有必要了解这些颗粒物引起的组织损伤。巨噬细胞主要参与机体对PM的反应，它们通过吞噬作用清除气道和肺部的颗粒。事实上，当巨噬细胞暴露于PM等有毒物质时，它们会经历一系列的变化（包括形态的变化、糖酵解的增加、随之而来的乳酸生成和白细胞介素-6和肿瘤坏死因子- α等细胞因子的释放），这些变化是将它们从“静止”转化为“活化”巨噬细胞所必需的。由于H-1核磁共振在无创监测巨噬细胞代谢方面非常有用，并且由于该技术从未被用于检测PM暴露后巨噬细胞的活化，因此本研究的目的是利用H-1核磁共振光谱研究PM暴露对RAW 264.7稳定巨噬细胞系的影响。带直径的PM
Because of the association between inhalation of airborne particulate matter (PM) and human respiratory and cardiovascular disease, it is necessary to understand the tissue damage induced by these particles. One of the cell types principally involved in the body's reaction to PM are macrophages, which remove particles in the airway passages and the lungs through phagocytosis. In fact, when macrophages are exposed to a toxic agent such as PM, they undergo a series of changes (including variations in morphology, an increase in glycolysis, and consequent lactate production and the release of cytokines such as interleukin-6 and tumor necrosis factor-alpha) necessary to transform them from "resting" to "activated" macrophages. Because H-1 NMR is extremely useful in monitoring, noninvasively, macrophage metabolism and because this technique has never been utilized to examine macrophage activation after exposure to PM, it was the purpose of the present study to investigate the effects of PM exposure on the RAW 264.7 stabilized macrophage cell line using H-1 NMR spectroscopy. PM with a diameter