Splicing enhances translation in mammalian cells: an additional function of the exon junction complex
Splicing enhances translation in mammalian cells: an additional function of the exon junction complex
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DOI：
10.1101/gad.1163204
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发表时间：
2004-01-15
影响因子：
10.5
通讯作者：
Moore, MJ
中科院分区：
文献类型：
--
作者：
Nott, A;Le Hir, H;Moore, MJ
在哺乳动物细胞中，剪接的mRNA比没有剪接的相同mRNA产生更多的蛋白质。这种增加的翻译产量与剪接mRNA的增强的细胞质多核糖体缔合相关，并且可归因于外显子连接复合物（EJC）的沉积。翻译刺激可以通过将EJC蛋白Y14、Magoh和RNPS1或无义介导的衰变（NMD）因子Upf1、Upf2和Upf3b拴系到无内含子报告mRNA来复制。因此，除了其在NMD中的先前表征的作用之外，EJC还促进mRNA多核糖体缔合。此外，在开放的阅读框架内刺激翻译的能力似乎是NMD所需因素的一般特征。
in mammalian cells, spliced mRNAs yield greater quantities of protein per mRNA molecule than do otherwise identical mRNAs not made by splicing. This increased translational yield correlates with enhanced cytoplasmic polysome association of spliced mRNAs, and is attributable to deposition of exon junction complexes (EJCs). Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA. Thus, in addition to its previously characterized role in NMD, the EJC also promotes mRNA polysome association. Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD.