Multiplexed short tandem repeat polymorphisms of the Weber 8A set of markers using tailed primers and infrared fluorescence detection.
Multiplexed short tandem repeat polymorphisms of the Weber 8A set of markers using tailed primers and infrared fluorescence detection.
复制标题
使用带尾引物和红外荧光检测对 Weber 8A 标记组进行多重短串联重复多态性分析。
DOI：
10.1002/elps.1150191806
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
King,RA
中科院分区：
文献类型：
--
作者：
Oetting,WS;Armstrong,CM;Ronan,SM;Young,TL;Sellers,TA;King,RA
短串联重复序列多态性（STRP）标记已成为遗传病定位的重要试剂。这些标记可用作筛选集，其以离散间隔位于所有染色体中，允许分析整个基因组。在分析中包括许多个体的作图研究需要产生大量的基因型。为了提高使用这些STRP筛选组的效率并降低其成本，我们将Weber 8A筛选组的扩增引物分成可以在单个聚合酶链式反应（PCR）扩增反应中扩增的组，从而减少了时间和成本。使用三引物反应产生荧光标记的扩增产物。每个标记的正向STRP扩增引物在5′端含有19 bp的序列。将具有与19 bp尾部相同的序列的荧光标记的引物作为荧光标记的唯一来源添加到扩增反应中。使用自动荧光DNA测序仪检测STRP带型。这种多重基因组筛选集的使用将大大增强人类疾病基因座的定位。
Short tandem repeat polymorphism (STRP) markers have become important reagents for mapping genetic diseases. These markers are available as screening sets, which are located in all chromosomes at discrete intervals, allowing the entire genome to be analyzed. Mapping studies that include many individuals in the analysis necessitate the production of large numbers of genotypes. In an effort to increase the efficiency and lower the cost of using these STRP screening sets, we have divided the amplification primers of the Weber 8A screening set into groups that can be amplified in single polymerase chain reaction (PCR) amplification reactions, resulting in a reduction of both time and cost. Fluorescently‐labeled amplification products were produced using a three primer reaction. The forward STRP amplification primer for each marker contained a 19 bp sequence at the 5′ end. A fluorescently‐labeled primer, with a sequence identical to the 19 bp tail, was added to the amplification reaction as the sole source of fluorescent label. The STRP banding pattern is detected using an automated fluorescent DNA sequencer. Use of this multiplexed genomic screening set should greatly enhance the mapping of human disease loci.