An electrochemical on-field sensor system for the detection of compost maturity
An electrochemical on-field sensor system for the detection of compost maturity
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DOI：
10.1016/j.aca.2006.08.030
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发表时间：
2007-01-09
影响因子：
6.2
通讯作者：
Tamiya, Eiichi
中科院分区：
文献类型：
--
作者：
Chikae, Miyuki;Kerman, Kagan;Tamiya, Eiichi
成熟度传感器系统的开发，基于三个电测参数，pH值，NH4+浓度，和磷酸酶活性的堆肥样品的水提取物的组合。这些参数之一，粗测试溶液中的表观磷酸酶活性使用在Nation膜中涂覆有α-磷酸萘酯（α-NP）的丝网印刷碳条（SPCS）测定。在SPCS上，用微分脉冲伏安法（DPV）用等份（30 μ L）供试品溶液监测磷酸酶活性。使用Tris-HCl缓冲液（pH 8.0）中的碱性磷酸酶（ALP）和柠檬酸缓冲液（pH 5.0）中的酸性磷酸酶（ACP）验证磷酸酶活性传感器。堆肥样品的水提取物中的加标酶的活性可以通过产物α-萘酚的相应氧化峰电流信号的变化来确定。在不同的堆肥日收集的堆肥样品（n = 24）的水提取物施加到我们的堆肥成熟度传感器系统，和常规的发芽试验。采用多元回归分析，将pH值、NH 4+浓度和磷酸酶活性组成多元线性回归方程，对发芽指数进行回归分析。由回归方程计算的GI与相应堆肥的实测GI具有良好的相关性（r = 0.873）。因此，我们已经确定了一个方程的堆肥稳定性的测定使用我们的便携式传感器系统迅速在堆肥现场。(c)2006 Elsevier B.V.保留所有权利。
A maturity sensor system was developed, based on the combination of three electrically measured parameters, pH, NH4+ concentration, and phosphatase activity in the water extracts of compost samples. One of these parameters, the apparent phosphatase activity in crude test solutions was determined using screen-printed carbon strips (SPCSs) coated with alpha-naphthyl phosphate (alpha-NP) in Nation film. The phosphatase activity was monitored in connection with differential pulse voltammetry (DPV) with an aliquot (30 mu L) of the test solution on SPCS. The phosphatase activity sensor was validated using alkaline phosphatase (ALP) in Tris-HCl buffer (pH 8.0) and acid phosphatase (ACP) in citric acid buffer (pH 5.0). The activity of the spiked enzymes in the water extract of the compost sample could be confirmed with the change of corresponding oxidation peak current signal of the product, alpha-naphthol. The water extracts of compost samples (n = 24) collected in various composting days were applied to our compost maturity sensor system, and the conventional germination tests. Using multiple regression analysis, the germination index (GI) was expressed by the multi-linear regression equation consisting of pH, NH4+ concentration, and the phosphatase activity. The calculated GI from the regression equation had a good correlation with the measured GI of the corresponding composts (r = 0.873). As a result, we have determined an equation for the determination of the compost stability using our portable sensor system rapidly at the composting site. (c) 2006 Elsevier B.V. All rights reserved.