Synthesis of a testosterone‐dependent secretory protein by rat seminal vesicle‐derived cell lines.
Synthesis of a testosterone‐dependent secretory protein by rat seminal vesicle‐derived cell lines.
复制标题
大鼠精囊来源的细胞系合成睾酮依赖性分泌蛋白。
作者：
G. Tajana;P. Locuratolo;S. Metafora;P. Abrescia;J. Guardiola
从大鼠精囊的外植体建立原代细胞培养物。除其他因素外，原代细胞培养物的建立需要睾酮的存在。在这种原代培养物中检测到两种细胞群：成纤维细胞样细胞和上皮样细胞；后者包含小细胞的亚型和大鳞状细胞的亚型（很可能是前者退化过程的结果）。组织化学以及电子显微镜观察表明，小上皮细胞存在持续的分泌活性。成纤维细胞和大鳞状上皮细胞在这方面不活跃。用过氧化物酶偶联抗体对细胞进行染色，并对标记蛋氨酸存在下产生的蛋白质进行分析，结果表明，还产生了一种主要的大鼠精囊分泌蛋白，即 RSV-IV。确定了有利于上皮细胞而不是成纤维细胞生长的条件。使用几乎同质的细胞群和使用胶原蛋白包被的培养皿，可以克隆两个独立获得的永久细胞系，即 SVC-1 和 SVC-2。两种细胞系的体外生长速率均受培养基中睾酮含量的调节。两种细胞系都能在睾酮控制下合成大量 RSV-IV 蛋白。
Primary cell cultures were established from explants of rat seminal vesicle. The establishment of primary cell cultures required, among other factors, the presence of testosterone. Two cell populations were detected in such primary cultures: fibroblast‐like cells and epithelial‐like cells; the latter encompassed a subtype of small cells and a subtype of large squamous cells (most likely the result of a degenerative process acting upon the former). Histochemical, as well as electron‐microscopical observations, indicated the presence of a persistent secretory activity in the small epithelial cells; fibroblast and large squamous epithelial cells were inactive in this respect. Staining of the cells with a peroxidase‐conjugated antibody and analysis of the proteins produced in the presence of labelled methionine, showed that one of the major rat seminal vesicle secretory proteins, namely RSV‐IV, was also produced. Conditions which favoured the growth of epithelial cells, rather than of fibroblasts, were determined. The use of nearly homogeneous cell populations and the use of collagen‐coated Petri dishes, allowed the cloning of two independently obtained permanent cell lines, namely SVC‐1 and SVC‐2. The in vitro growth rate of both cell lines was modulated by the amount of testosterone in the medium. Both cell lines were able to synthesize a significant amount of RSV‐IV protein under testosterone control.