IDENTIFICATION OF RHIZOBIUM STRAINS IN PEA ROOT NODULES USING GENETIC MARKERS
IDENTIFICATION OF RHIZOBIUM STRAINS IN PEA ROOT NODULES USING GENETIC MARKERS
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DOI：
10.1099/00221287-87-2-343
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
BERINGER, JE
中科院分区：
文献类型：
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作者：
JOHNSTON, AWB;BERINGER, JE
豌豆植株与根瘤菌的遗传标记菌株对联合接种，在检测的297个根瘤中，56个含有两种接种菌株。接种物中菌株的比例不影响混合根瘤的频率。一般来说，其中一种菌株始终占据大多数结核，在混合结核中，细菌占大多数。转移的P-组R因子，RP 4，在混合根瘤内的某些根瘤菌菌株之间进行了检测。在某些情况下，非亲本后代包含10%的从这些根瘤中分离的根瘤菌。
Pea plants were inoculated jointly with pairs of genetically marked strains ofRhizobium leguminosarum.Out of 297 nodules examined 56 contained both inoculant strains. The ratios of the strains in the inoculum did not affect the frequencies of mixed nodules. Generally one of the strains consistently occupied the majority of the nodules and in the mixed nodules comprised the majority of bacteria. Transfer of the P-group R factor, RP4, between certain strains of Rhizobium within mixed nodules was detected. In some cases the non-parental progeny comprised 10 % of the rhizobia isolated from such nodules.