ARTHROPATHIC PROPERTIES OF CELL-WALL POLYMERS FROM NORMAL FLORA BACTERIA
ARTHROPATHIC PROPERTIES OF CELL-WALL POLYMERS FROM NORMAL FLORA BACTERIA
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DOI：
10.1128/iai.51.1.240-249.1986
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发表时间：
1986-01-01
影响因子：
3.1
通讯作者：
SCHWAB, JH
中科院分区：
文献类型：
--
作者：
STIMPSON, SA;BROWN, RR;SCHWAB, JH
从D组链球菌(粪链球菌，ATCC 9790和F-24菌株)的细胞壁中提纯了肽聚糖-多糖(PG-PS)片段，其方法是最大限度地减少自溶活性，并测试了其诱导大鼠关节炎的能力。其他正常菌群细菌(产消化链球菌和痤疮丙酸杆菌)、A组链球菌的Pg-PS片段和甲酸甲烷杆菌细胞壁的Pg-PS片段也同样进行了纯化和检测。所有PG-PS聚合物关节内注射到大鼠脚踝后，均可引起急性炎症；假性尿毒素-PS片段的活性约为PG-PS制剂的五分之一。腹腔感染后，痤疮巴氏杆菌PG-PS诱导轻微急性关节炎，PG-PS诱导中重度急性关节炎症，随后轻度慢性关节炎，A、D两组链球菌PG-PS均诱导严重急性关节炎，演变为慢性侵蚀性关节疾病；假尿毒素-PS片段无效，与PG-PS的PG部分起关键作用一致。D组链球菌PG-PS诱导的慢性关节炎在60d后消退，而A组链球菌PG-PS结构来源于正常菌群诱导的慢性关节炎有可能诱发从一过性急性到慢性侵蚀性关节疾病的广泛反应。
Peptidoglycan-polysaccharide (PG-PS) fragments were purified from cell walls of group D streptococci (Steptococcus faecium, stains ATCC 9790 and F-24) with a protocol which minimizes autolytic activity and tested for ability to induce arthritis in rats. PG-PS fragments from cell walls of other normal flora bacteria (Peptostreptococcus productus, and Propionibacterium acnes), group A streptococci, and pseudomurein-PS fragments from cell walls of Methanobacterium formicicum, were similarly purified and tested. Upon intraarticular injection into rat ankles, all PG-PS polymers induced acute inflammation; pseudomurein-PS fragments were approximately five times less active than the PG-PS preparations. After intraperitoneal infection, P. acnes PG-PS induced a minimal acute arthritis Peptostreptococcus productus PG-PS induced a moderately severe acute joint inflammation followed by a mild chronic arthritis, and both group A and group D streptococcal PG-PS induced severe acute arthritis which evolved into chronic, erosive joint disease; pseudomurein-PS fragments were without effect, consistent with a crucial role for the PG moiety of PG-PS. Chronic arthritis induced by group D streptococcal PG-PS subsided after 60 days, whereas that induced by group A streptococcal PG-PS structures derived from the normal flora have the potential to induce a wide range of responses, from transient acute to chronic erosive joint disease.