Identification of candidate genes involved in the radiosensitivity of esophageal cancer cells by microarray analysis
Identification of candidate genes involved in the radiosensitivity of esophageal cancer cells by microarray analysis
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DOI：
10.1111/j.1442-2050.2007.00759.x
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Fujii, Y.
中科院分区：
文献类型：
--
作者：
Ogawa, R.;Ishiguro, H.;Fujii, Y.
放射治疗在控制食道癌患者肿瘤生长中起着关键作用。要确定哪些患者将从放射治疗中受益最多，了解与食道癌细胞辐射敏感性有关的基因是很重要的。因此，我们检测了辐射敏感和辐射抵抗的食管鳞癌细胞系中的整体基因表达。测定了13株食管癌细胞系的放射敏感性。从每个食道癌细胞系和一个正常的食道上皮细胞系中提取RNA，利用34594个点的寡核苷酸芯片分析全球基因表达谱。在克隆形成实验中，一个细胞系(TE-11)被鉴定为对辐射高度敏感，而其他细胞系被发现相对抗辐射。通过基因芯片分析，我们筛选出了在TE-11中差异表达的71个候选基因。上调的基因包括CABPR、FABP5、DSC2、GPX2、NME、CBR3、DOCK8和ABCC5，下调的基因包括RPA1、LDOC1、NDN和SKP1A。我们的研究提供了与食道癌细胞辐射敏感性相关的基因的全面信息，这些信息可以作为进一步功能研究的基础。
Radiotherapy plays a key role in the control of tumor growth in esophageal cancer patients. To identify the patients who will benefit most from radiation therapy, it is important to know the genes that are involved in the radiosensitivity of esophageal cancer cells. Hence, we examined the global gene expression in radiosensitive and radioresistant esophageal squamous cell carcinoma cell lines. Radiosensitivities of 13 esophageal cancer cell lines were measured. RNA was extracted from each esophageal cancer cell line and a normal esophageal epithelial cell line, and the global gene expression profiles were analyzed using a 34 594-spot oligonucleotide microarray. In the clonogenic assay, one cell line (TE-11) was identified to be highly sensitive to radiation, while the other cell lines were found to be relatively radioresistant. We identified 71 candidate genes that were differentially expressed in TE-11 by microarray analysis. The up-regulated genes included CABPR, FABP5, DSC2, GPX2, NME, CBR3, DOCK8, and ABCC5, while the down-regulated genes included RPA1, LDOC1, NDN, and SKP1A. Our investigation provided comprehensive information on genes related to radiosensitivity of esophageal cancer cells; this information can serve as a basis for further functional studies.