Bone marrow- and adipose-derived stem cells show expression of myelin mRNAs and proteins
Bone marrow- and adipose-derived stem cells show expression of myelin mRNAs and proteins
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DOI：
10.2217/rme.10.15
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发表时间：
2010-05-01
影响因子：
2.7
通讯作者：
Wiberg, Mikael
中科院分区：
文献类型：
--
作者：
Mantovani, Cristina;Mahay, Daljeet;Wiberg, Mikael
目的：PNS髓磷脂是由雪旺细胞（SCs）形成的。在本研究中，我们利用骨髓间充质干细胞和脂肪来源的干细胞分化成sc样细胞，并与分离的成人背根神经节神经元共培养，建立了体外模型来研究髓磷脂的形成。方法：应用免疫细胞化学、逆转录pcr和western blotting技术在转录和翻译水平上研究髓磷脂蛋白的表达。结果：在与神经细胞共培养的分化干细胞中检测到蛋白0、外周髓鞘蛋白22和髓鞘碱性蛋白的转录本。此外，在共培养中还能识别出蛋白0、外周髓鞘蛋白22和髓鞘碱性蛋白。这些结果与髓磷脂蛋白免疫染色和电镜观察一致。结论：两种分化为sc样细胞的成体干细胞在再生过程中都有可能成为髓鞘神经细胞，其功能与PNS的sc相同。
Aims: PNS myelin is formed by Schwann cells (SCs). In this study, we applied an in vitro model to study myelin formation, using bone marrow mesenchymal stem cells and adipose-derived stem cells differentiated into SC-like cells and co-cultured with dissociated adult dorsal root ganglia neurons. Methods: Immunocytochemistry, reverse transcription-PCR and western blotting techniques were used to investigate the expression of myelin proteins at both the transcriptional and translational level. Results: Transcripts for protein zero, peripheral myelin protein 22 and myelin basic protein were detected in differentiated stem cells following co-culture with neuronal cells. Furthermore, protein zero, peripheral myelin protein 22 and myelin basic proteins were recognized in the co-cultures. These results were consistent with immunostaining of myelin proteins and with observation by electron microscopy. Conclusion: Both types of adult stems cells differentiated into SC-like cells have potential to myelinate neuronal cells during regeneration, being functionally identical to SCs of the PNS.