Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles
Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles
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DOI：
10.1126/science.277.5329.1078
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发表时间：
1997-08-22
期刊：
影响因子：
56.9
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Elghanian, R;Storhoff, JJ;Mirkin, CA
报道了一种基于硫醇-烷基寡核苷酸修饰的金纳米粒子探针的高选择性比色多核苷酸检测方法。将单链目标寡核苷酸(30个碱基)引入含有适当探针的溶液中，可形成由纳米颗粒组成的聚合物网络，并伴随着从红色到粉红色/紫色的颜色变化。溶液的冷冻和解冻促进了杂交，这些杂化材料的变性在一个狭窄的范围内显示出转变温度，允许区分各种不完美的靶标。将杂交混合物转移到反相硅胶板上，干燥后产生蓝色，可以用肉眼检测到。未经优化的系统可以检测到一个寡核苷酸的大约10个毫微摩尔。
A highly selective, colorimetric polynucleotide detection method based on mercaptoal-kyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into a solution containing the appropriate probes resulted in the formation of a polymeric network of nanoparticles with a concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing of the solutions, and the denaturation of these hybrid materials showed transition temperatures over a narrow range that allowed differentiation of a variety of imperfect targets. Transfer of the hybridization mixture to a reverse-phase silica plate resulted in a blue color upon drying that could be detected visually. The unoptimized system can detect about 10 femtomoles of an oligonucleotide.