Crystallization of bacteriorhodopsin solubilized by a tripod amphiphile
Crystallization of bacteriorhodopsin solubilized by a tripod amphiphile
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DOI：
10.1016/j.bbapap.2005.04.011
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发表时间：
2005-08-10
影响因子：
3.2
通讯作者：
Chiu, ML
中科院分区：
文献类型：
--
作者：
Theisen, MJ;Potocky, TB;Chiu, ML
细菌视紫红质(W)作为一种单体被一种新型的表面活性剂三脚架两亲性(TPA)有效地溶解，它允许在几种条件下形成紫色的六方BR晶体。这些晶体虽然很小，但使用同步辐射可以衍射到2.5埃的分辨率。TPA可用于其他膜蛋白的增溶、纯化和结晶。(C)2005 Elsevier B.V.保留所有权利。
Bacteriorhodopsin (W) is solubilized efficiently as a monomer by a novel surfactant, a tripod amphiphile (TPA), which permits the formation of purple hexagonal bR crystals under several conditions. The crystals, although small, diffract to 2.5 angstrom resolution using synchrotron radiation. TPA may be useful for the solubilization, purification, and crystallization of other membrane proteins. (c) 2005 Elsevier B.V. All rights reserved.