CDNA FOR THE HUMAN BETA-2-ADRENERGIC RECEPTOR - A PROTEIN WITH MULTIPLE MEMBRANE-SPANNING DOMAINS AND ENCODED BY A GENE WHOSE CHROMOSOMAL LOCATION IS SHARED WITH THAT OF THE RECEPTOR FOR PLATELET-DERIVED GROWTH-FACTOR
CDNA FOR THE HUMAN BETA-2-ADRENERGIC RECEPTOR - A PROTEIN WITH MULTIPLE MEMBRANE-SPANNING DOMAINS AND ENCODED BY A GENE WHOSE CHROMOSOMAL LOCATION IS SHARED WITH THAT OF THE RECEPTOR FOR PLATELET-DERIVED GROWTH-FACTOR
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DOI：
10.1073/pnas.84.1.46
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发表时间：
1987-01-01
影响因子：
11.1
通讯作者：
LEFKOWITZ, RJ
中科院分区：
文献类型：
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作者：
KOBILKA, BK;DIXON, RAF;LEFKOWITZ, RJ
我们已经分离并测序了一个编码人类β的cDNA。2-adrenergic受体。推导出的氨基酸序列（413个残基）是一个含有7个疏水氨基酸簇的蛋白质，提示其具有跨膜结构域。虽然蛋白质与先前克隆的仓鼠有87%的相同。2-肾上腺素能受体，最高度保守的区域是假定的跨膜螺旋（95%相同）和细胞质环（93%相同），这表明这些区域的分子具有重要的功能域。一些跨膜螺旋也与视觉色素的视蛋白家族成员的可比区域具有较小程度的同一性。我们找到了。β的基因。-肾上腺素能受体到第5染色体上的q31-q32。这与最近确定的编码血小板衍生生长因子受体的基因的位置相同，并且与编码巨噬细胞集落刺激因子受体的FMS原癌基因的位置相邻。
We have isolated and sequence a cDNA encoding the human .beta.2-adrenergic receptor. The deduced amino acid sequence (413 residues) is that of a protein containing seven clusters of hydrophobic amino acids suggestive of membrane-spanning domains. While the protein is 87% identical overall with the previously clones hamster .beta.2-adrenergic receptor, the most highly conserved regions are the putative transmembrane helices (95% identical) and cytoplasmic loops (93% identical), suggestin that these regions of the molecular harbor important functional domains. Several of the transmembrane helices also share lesser degrees of identity with comparable regions of select members of the opsin family of visual pigments. We have localized the gene for the .beta.-adrenergic receptor to q31-q32 on chromosome 5. This is the same position recently determined for the gene encoding the receptor for platelet-derived growth factor and is adjacent to that for the FMS protooncogene, which encodes the receptor for the macrophage colony-stimulating factor.