Optogenetic dissection of descending behavioral control in Drosophila.
Optogenetic dissection of descending behavioral control in Drosophila.
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DOI：
10.7554/elife.34275
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发表时间：
2018-06-26
期刊：
影响因子：
7.7
通讯作者：
Berman GJ
中科院分区：
文献类型：
--
作者：
Cande J;Namiki S;Qiu J;Korff W;Card GM;Shaevitz JW;Stern DL;Berman GJ
在大多数动物中，大脑做出的行为决定由下行神经元传递到产生行为的神经索电路。在昆虫中，只有少数下行神经元与特定行为有关。为了探索下行神经元如何控制昆虫的运动，我们开发了一种新的方法来系统地测试激活单个神经元对自由行为的陆地黑腹果蝇的行为影响。我们计算了这些苍蝇探索的整个行为空间的二维表示，并通过识别该空间中在光遗传激活期间访问频率增加的区域，将下行神经元与特定行为联系起来。将这一方法应用于大量下行神经元，我们发现(1)大多数下行神经元的激活驱动刻板行为，(2)在许多情况下，多个下行神经元激活类似的行为，以及(3)光遗传激活的行为通常取决于激活前的行为状态。
In most animals, the brain makes behavioral decisions that are transmitted by descending neurons to the nerve cord circuitry that produces behaviors. In insects, only a few descending neurons have been associated with specific behaviors. To explore how descending neurons control an insect’s movements, we developed a novel method to systematically assay the behavioral effects of activating individual neurons on freely behaving terrestrial D. melanogaster. We calculated a two-dimensional representation of the entire behavior space explored by these flies, and we associated descending neurons with specific behaviors by identifying regions of this space that were visited with increased frequency during optogenetic activation. Applying this approach across a large collection of descending neurons, we found that (1) activation of most of the descending neurons drove stereotyped behaviors, (2) in many cases multiple descending neurons activated similar behaviors, and (3) optogenetically activated behaviors were often dependent on the behavioral state prior to activation.