[Determination of benzimidazole anthelmintics in livestock foods by HPLC].
[Determination of benzimidazole anthelmintics in livestock foods by HPLC].
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高效液相色谱法测定畜禽食品中苯并咪唑类驱虫药的含量[J].
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
H. Nakazawa
中科院分区：
文献类型：
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作者：
K. Takeba;K. Fujinuma;M. Sakamoto;Katsuhiko Jimbo;H. Oka;Yuko Ito;H. Nakazawa
建立了一种同时测定畜禽食品中12种苯并咪唑类驱虫剂的反相高效液相色谱-光电二极管阵列检测器（PDA）方法。用乙腈和正己烷均质化样品，并离心。分离乙腈相并蒸发。将残留物溶于0.1 mol/L碳酸盐缓冲液（pH = 9.1）中，超声处理，然后在Bond Elut LRC-C18柱上进行净化。将苯并咪唑化合物在Capcell Pak C18 UG 120（5微米，150 × 4.6 mm i.d.）用PDA在295和313 nm处检测。移动的相为乙腈和0.05mol/L乙酸铵（20：80和40：60），流速为1.0mL/min，检测温度为40 ℃。在0.1-0.5 μ g/g的加样量范围内，平均回收率（n = 3）为72.6-97.2%，变异系数为0.3- 8.5%。检出限为0.01-0.05 μ g/g。
A simple and rapid liquid chromatographic method was developed for the simultaneous determination of twelve benzimidazole anthelmintics in livestock foods using reversed-phase high-performance liquid chromatography with photodiode array detection (PDA). A sample was homogenized with acetonitrile and n-hexane, and centrifuged. The acetonitrile phase was isolated and evaporated. The residue was dissolved in 0.1 mol/L carbonate buffer solution (pH = 9.1), sonicated, and then subjected to clean-up on a Bond Elut LRC-C18 cartridge. The benzimidazole compounds were separated isocratically on a Capcell Pak C18 UG 120 (5 microns, 150 x 4.6 mm i.d.) column and detected by PDA at 295 and 313 nm. Mixtures of acetonitrile and 0.05 mol/L ammonium acetate in mixing ratios of (20:80) and (40:60) were used as the mobile phase, and the flow-rate was 1.0 mL/min at 40 degrees C. The mean recoveries (n = 3) from 0.1-0.5 microgram/g added samples were 72.6-97.2% with coefficients of variation of 0.3-8.5%. The detection limits were 0.01-0.05 microgram/g.
牛奶中7种苯并咪唑类驱虫药多残留分离及液相色谱测定方法
DOI：
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发表时间：
1989
期刊：
Journal - Association of Official Analytical Chemists
影响因子：
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作者：
Long,AR;Hsieh,LC;Malbrough,MS;Short,CR;Barker,SA
通讯作者：
Barker,SA