Early photolysis intermediates of the artificial visual pigment 13-demethylrhodopsin.
Early photolysis intermediates of the artificial visual pigment 13-demethylrhodopsin.
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人工视觉色素 13-去甲基视紫红质的早期光解中间体。
DOI：
10.1021/bi00458a020
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Kliger,DS
中科院分区：
文献类型：
--
作者：
Einterz,CM;Hug,SJ;Lewis,JW;Kliger,DS
加州大学圣克鲁斯分校化学系，1989年6月6日收到；修订稿收到1989年10月3日摘要：报道了一种含有生色团13-去甲基视紫红质的修饰视紫红质色素的室温光解的纳秒时间分辨吸收测量。这些测量结果与BA-THO-13-去甲基视紫红质物种和蓝移物种(相对于母体色素)BSI-13-去甲基视紫红质之间的平衡形成一致。这些结果与天然牛视紫质的光解[Hug，S.J.，Lewis，J.W.，Einterz，C.M.，Thorgeirsson，Thorgeirsson，&Kriger，D.S.(1990)生物化学(本期的前一篇论文)]以及几种首次观察到BSI物种的改性等视紫红质色素光解后的结果进行了比较。结果表明，在所有的颜料中，结果与Batho和Bsi之间形成的平衡是一致的，Bsi随后在室温下以纳秒的时间尺度衰减到光视紫红质中间体。
Chemistry Department, University of California, Santa Cruz, California 95064 Received June 6, 1989; Revised Manuscript Received October 3, 1989 abstract: Nanosecond time-resolved absorption measurements are reported for the room temperature photolysis of a modified rhodopsin pigment, 13-demethylrhodopsin, which contains the chromophore 13-demethylretinal. Themeasurements are consistent with the formation of an equilibrium between a BA-THO-13-demethylrhodopsin species and a blue-shifted species (relative to the parent pigment), BSI-13-demethylrhodopsin. The results are compared to those acquired after photolysis of native bovine rhodopsin [Hug, S. J., Lewis, J. W., Einterz, C. M., Thorgeirsson, T. E., & Kliger, D. S.(1990) Biochemistry (preceding paper in this issue)] and to results obtained after photolysis of several modified isorhodopsin pigments in which the BSI species was first observed. It is concluded that in all of the pigments the results are consistent with the formation of an equilibrium between BATHO and BSI, which subsequently decays on a nanosecond time scale at room temperature to a lumirhodopsin intermediate.