Influence of molecular weight on oral absorption of water soluble chitosans
Influence of molecular weight on oral absorption of water soluble chitosans
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DOI：
10.1016/j.jconrel.2004.10.012
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发表时间：
2005-02-02
影响因子：
10.8
通讯作者：
Nah, JW
中科院分区：
文献类型：
--
作者：
Chae, SY;Jang, MK;Nah, JW
壳聚糖是一种阳离子多糖，广泛用作膳食补充剂以及在药理和生物医学方面的应用。虽然许多研究都集中在它作为药物辅料或生物活性试剂的应用上，但依赖于分子量(MW)的药物和生物活性性质仍然不清楚。作为初步研究，我们研究了口服水溶性壳聚糖(WSCs)后依赖NW的Caco-2细胞层转运现象(体外)和肠道吸收模式(体内)。壳聚糖的相对分子质量对其吸收有显著影响。随着相对分子质量的增加，吸收减小。与高相对分子质量壳聚糖(WSC 230K，Mw=230 kDa)相比，WSC 3.8K(Mw=3.8 kDa)在体外和体内的吸收峰分别增加了23倍和25倍以上。此外，壳聚糖具有浓度和相对分子质量依赖性的细胞毒作用，壳寡糖(MW
Chitosan, a cationic polysaccharide, has been widely employed as dietary supplement and in pharmacological and biomedical applications. Although numerous studies have focused on its applications as pharmaceutical excipients or bioactive reagents, molecular weight (MW)-dependent pharmaceutical and bioactive properties remain unclear. As a preliminary study, we investigated the NW-dependent Caco-2 cell layer transport phenomena (in vitro) and intestinal absorption patterns after oral administration (in vivo) of water-soluble chitosans (WSCs). The absorption of chitosan was significantly influenced by its MW. As the MW increases, the absorption decreases. Compared to high-MW chitosan (WSC 230K, MW=230 kDa), absorption profiles were observed to increase more than 23 and 25 times with WSC 3.8K (MW=3.8 kDa) in both in vitro and in vivo transport experiments, respectively. Furthermore, the chitosans showed concentration- and MW-dependent cytotoxic effects, and the chitosan oligosaccharides (MW