MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE PESTIVIRUS BOVINE VIRAL DIARRHEA VIRUS
MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE PESTIVIRUS BOVINE VIRAL DIARRHEA VIRUS
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DOI：
10.1016/0042-6822(88)90672-1
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发表时间：
1988-07-01
期刊：
影响因子：
3.7
通讯作者：
PURCHIO, AF
中科院分区：
文献类型：
--
作者：
COLLETT, MS;LARSON, R;PURCHIO, AF
对牛病毒性腹泻病毒（BVDV）致细胞病变NADL分离株的RNA基因组进行了分子克隆，并测定了核苷酸序列。克隆的序列长12，573个核苷酸，对应于4.3 ×的分子量106，具有32.2%A、25.7%G、22.1%U和20.0%C的基础组成。然而，RNA的5“和3”末端的序列尚未明确确定。在病毒有义（正极性）序列中发现延伸分子长度的单个主要开放阅读框。该开放阅读框编码3988个氨基酸，代表449 kDa的蛋白质。
The RNA genome of the cytopathic NADL isolate of bovine viral diarrhea virus (BVDV) has been molecularly cloned and the nucleotide sequence determined. The cloned sequence was 12,573 nucleotides in length, corresponding to a molecular weight of 4.3 .times. 106, having a base composition of 32.2% A, 25.7% G, 22.1% U, and 20.0% C. However, the sequences at the 5'' and 3'' termini of the RNA have not been unequivocally established. A single major open reading frame extending the length of the molecule was found in the viral-sense (positive polarity) sequence. This open reading frame was capable of encoding 3988 amino acids, representing 449 kDa of protein.