Silkworm TFIIIA requires additional class III factors for commitment to transcription complex assembly on a 5S RNA gene.
Silkworm TFIIIA requires additional class III factors for commitment to transcription complex assembly on a 5S RNA gene.
复制标题
蚕 TFIIIA 需要额外的 III 类因子来参与 5S RNA 基因上的转录复合物组装。
DOI：
10.1093/nar/23.7.1244
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发表时间：
1995
影响因子：
14.9
通讯作者：
Sprague,KU
中科院分区：
文献类型：
--
作者：
Smith,TP;Young,LS;Bender,LB;Sprague,KU
摘要我们发现家蚕5S RNA和tRNA基因在启动子结构和模板定型要求上有惊人的相似性。启动子是几乎相同的大小（约160 bp），包括侧翼以及内部sequences.To分析的因素要求5S RNA转录复合物组装在一个完全同源的系统，我们已经分离出的家蚕馏分是高度富集的5S RNA特异性转录因子，TFIIIA。使用该馏分，连同其他蚕馏分，TFIIIB，TFIIIC，TFIIID和RNA聚合酶III，我们证明了5S RNA转录复合物组装的要求是非常相似的那些先前建立的tRNACAlagene。具体地说，没有单个因子分数是足够的承诺家蚕5S RNA基因转录复合物组装。相反，需要至少三个因子分数的组合。我们的观察表明，一个以上的子集的因素是有能力的承诺表明，家蚕5S RNA基因进一步类似于tRNAcAlagenes在他们的能力，使用多种途径的转录复合物的形成。
ABSTRACTWe find striking similarities In promoter structure and requirements for template commitment on 5S RNA and tRNA genes from silkworms. The promoters are nearly the same size (∼160 bp) and include flanking as well as internal sequences.To analyze the factor requirements for 5S RNA transcription complex assembly in a completely homologous system, we have isolated a silkworm fraction that is highly enriched for the 5S RNA-specific transcription factor, TFIIIA. Using this fraction, together with the other silkworm fractions, TFIIIB, TFIIIC, TFIIID and RNA polymerase III, we demonstrate that the requirements for 5S RNA transcription complex assembly are very similar to those previously established for a tRNACAlagene. Specifically, no individual factor fraction is sufficient for commitment of silkworm 5S RNA genes totranscription complex assembly. Rather, combinations of at least three factor fractions are required. Our observation thatmore than one subset of factors is competent for commitment suggests that silkworm 5S RNA genes further resemble tRNAcAlagenes in their ability to use multiple pathways for transcription complex formation.