Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs
Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs
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蓖麻子蜱蓖麻硬蜱的蛋白质组学分析：关注化学感应器官
DOI：
10.1016/j.ibmb.2016.09.004
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发表时间：
2016-11-01
影响因子：
3.8
通讯作者：
Dani, Francesca Romana
中科院分区：
文献类型：
--
作者：
Iovinella, Immacolata;Ban, Liping;Dani, Francesca Romana
在节肢动物中，绝大多数关于嗅觉的研究都集中在昆虫身上，其中大多数涉及的蛋白质已经被鉴定出来。特别地，昆虫中的化学感受依赖于两个家族的膜受体，嗅觉/味觉受体（OR/GR）和离子型受体（IR），以及两类可溶性蛋白，气味结合蛋白（OBP）和化学感受蛋白（CSP）。在其他节肢动物中，如蜱和螨，只有IR已被确定，而编码OBP和CSP的基因缺失。第三类可溶性蛋白，称为尼曼-匹克C2（NPC 2）已被建议作为潜在的载体的信息化学物质在昆虫和其他arthropods.here我们报告的蛋白质组学分析的结果的嗅觉器官（Haller的器官和触须）和控制组织的蜱蜱蓖麻，和免疫染色实验针对NPC 2s。采用不同的提取和蛋白质组学方法，我们确定了大量的蛋白质，并强调了差异表达。另一方面，利用免疫细胞化学方法，我们在Haller's器官和触须感受器中检测到针对其中一个NPC 2的反应。我们推测，蜱组织中这种蛋白质的低浓度可能解释了不一致的结果。在配体结合试验中，相应的重组NPC 2表现出良好的亲和力，荧光探针N-苯基萘胺和一些有机化合物，支持推定的作用NPC 2作为气味载体。(C)2016爱思唯尔有限公司版权所有
In arthropods, the large majority of studies on olfaction have been focused on insects, where most of the proteins involved have been identified. In particular, chemosensing in insects relies on two families of membrane receptors, olfactory/gustatory receptors (ORs/GRs) and ionotropic receptors (IRs), and two classes of soluble proteins, odorant-binding proteins (OBPs) and chemosensory proteins (CSPs). In other arthropods, such as ticks and mites, only IRs have been identified, while genes encoding for OBPs and CSPs are absent. A third class of soluble proteins, called Niemann-Pick C2 (NPC2) has been suggested as potential carrier for semiochemicals both in insects and other arthropods.Here we report the results of a proteomic analysis on olfactory organs (Haller's organ and palps) and control tissues of the tick Ixodes ricinus, and of immunostaining experiments targeting NPC2s. Adopting different extraction and proteomic approaches, we identified a large number of proteins, and highlighted those differentially expressed. None of the 13 NPC2s known for this species was found. On the other hand, using immunocytochemistry, we detected reaction against one NPC2 in the Haller's organ and palp sensilla. We hypothesized that the low concentration of such proteins in the tick's tissues could possibly explain the discrepant results. In ligand-binding assays the corresponding recombinant NPC2 showed good affinity to the fluorescent probe N-phenylnaphthylamine and to few organic compounds, supporting a putative role of NPC2s as odorant carriers. (C) 2016 Elsevier Ltd. All rights reserved.