Quantitative determination of sulphur containing amino acids in various globine
Quantitative determination of sulphur containing amino acids in various globine
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DOI：
10.1515/bchm2.1940.265.2-3.94
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发表时间：
1940-01-01
影响因子：
--
通讯作者：
Taurins, A
中科院分区：
文献类型：
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作者：
Birkofer, L;Taurins, A
根据Kuhn、Birkhofer和Quackenbush（Ber. [医]化学72：407，1939）。为了获得CO-Hb，将溶血溶液离心血液以除去基质，并用200 cc的CO2饱和。的CO-Hb溶液。与100 cc混合。2 M磷酸盐缓冲液，置于玻璃纸袋中，用3.6M磷酸盐缓冲溶液透析。5-6度。通过倾倒O2-或CO-Hb溶液获得球蛋白。10-20卷含有0.5%HCl的丙酮。蛋氨酸和胱氨酸被检测到。根据Kuhn、Birkhofer和Quackenbush的方法。（Ber. [医]化学72：407，1939.）年龄也不。性别、血型和疾病均不影响人的蛋氨酸和胱氨酸值，人血红蛋白中蛋氨酸含量为5.98-6.54摩尔/66,000克。血红蛋白和2.80-3.56摩尔胱氨酸。在恒河猴中也发现了类似的数值。牛的蛋氨酸为7.55-8.35，胱氨酸为1.24-1.85;马为3.93-4.54，胱氨酸为1.79-2.57;狗、狐狸和豺为2.34-2.60，胱氨酸为4.30-4.75。
Crystalline O2-Hb was prepared according to the method of Kuhn, Birkhofer, and Quackenbush (Ber. chem. Ges. 72: 407, 1939). To obtain CO-Hb, the hemolyzed soln. of blood was centrifugalized to remove stroma, and saturated with CO. 200 cc. of the CO-Hb soln. were mixed with 100 cc. 2M phosphate buffer soln., placed in a cellophane bag and dialyzed against 3.6M phosphate buffer soln. at 5-6[degree]. The globins were obtained by pouring the O2- or CO-Hb solns. into 10-20 vols. acetone which contained 0.5% HC1. Methionine and cystine were det. according to the method of Kuhn, Birkhofer and Quackenbush. (Ber. chem. Ges. 72: 407, 1939.) Neither age. sex, blood groups nor sickness affected the methionine and cystine values for man. Human Hb contained 5.98-6.54 moles of methionine per 66,000 g. of Hb, and 2.80-3.56 moles of cystine. Similar values were found for rhesus monkeys. In cattle, methionine was 7.55-8.35 and cystine 1.24-1.85; in horses, 3.93-4.54, and 1.79-2.57; in dog, fox and jackel, 2.34-2.60 and 4.30-4.75.