SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis
SpHnf6, a transcription factor that executes multiple functions in sea urchin embryogenesis
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DOI：
10.1016/j.ydbio.2004.05.033
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发表时间：
2004-09-15
影响因子：
2.7
通讯作者：
Davidson, EH
中科院分区：
文献类型：
--
作者：
Otim, O;Amore, G;Davidson, EH
紫球海胆hnf6（Sphnf6）基因编码ONECUT转录因子家族的新成员。hnf6在发育胚胎中的表达是三相的，功能丧失分析表明，Hnf6蛋白是一种转录因子，在海胆发育中具有多种不同的作用。hnf6在母体中表达，并且在原肠胚形成之前其转录物在全球分布。在发育早期，其表达是PMC分化基因如sm50、pm27和msp 130的激活所需的，但不是任何已知PMC调节基因如alx、ets1、pmar 1或tbrain的激活所需的。微球移植实验表明，该基因不参与早期微球信号。早期hnf6的表达也是中胚层调节因子gatac表达所必需的。hnf6的第二个已知作用是其在原肠胚形成后参与口腔外胚层基因调控网络（GRN），其中其表达对于维持口腔外胚层特化状态至关重要。第三个作用是在神经源性纤毛带中，这是由hnf6在口腔外胚层边缘表达的梯形带所预示的，并且在胚胎发生结束时继续表达。在没有hnf 6表达的情况下，口腔外胚层的调节功能和纤毛带的形成都不会正常发生。(C)2004年爱思唯尔公司All rights reserved.
The Strongylocentrotus purpuratus hnf6 (Sphnf6) gene encodes a new member of the ONECUT family of transcription factors. The expression of hnf6 in the developing embryo is triphasic, and loss-of-function analysis shows that the Hnf6 protein is a transcription factor that has multiple distinct roles in sea urchin development. hnf6 is expressed maternally, and before gastrulation its transcripts are distributed globally Early in development, its expression is required for the activation of PMC differentiation genes such as sm50, pm27, and msp130, but not for the activation of any known PMC regulatory genes, for example, alx, ets1, pmar1, or tbrain. Micromere transplantation experiments show that the gene is not involved in early micromere signaling. Early hnf6 expression is also required for expression of the mesodermal regulator gatac. The second known role of hnf6 is its participation after gastrulation in the oral ectoderm gene regulatory network (GRN), in which its expression is essential for the maintenance of the state of oral ectoderm specification. The third role is in the neurogenic ciliated band, which is foreshadowed exactly by a trapezoidal band of hnf6 expression at the border of the oral ectoderm and where it continues to be expressed through the end of embryogenesis. Neither oral ectoderm regulatory functions nor ciliated band formation occur normally in the absence of hnf6 expression. (C) 2004 Elsevier Inc. All rights reserved.