3-epicabraleabydroxylactone and other triterpenoids from camellia oil and their inhibitory effects on Epstein-Barr virus activation
3-epicabraleabydroxylactone and other triterpenoids from camellia oil and their inhibitory effects on Epstein-Barr virus activation
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DOI：
10.1248/cpb.52.153
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发表时间：
2004-01-01
影响因子：
1.7
通讯作者：
Nishino, H
中科院分区：
文献类型：
--
作者：
Akihisa, T;Tokuda, H;Nishino, H
基于光谱和化学方法，从山茶花（Camellia japonica L.；Theaccae）种子油的不皂化脂质（NSL）中分离出的三萜化合物的结构为（20S）-3β-羟基-25,26,27-trisnordammaran-24,20-olide（1; 3-epicabraleaHydroxylactone）。从 NSL 中分离出的其他六种三萜类化合物被鉴定为 3-epicabraleadiol (2)、ocotillol II (3)、ocotillol I (4)、dammarenediol II (5)、(20R)-taraxastane-3beta,20-diol (6) 和 lupane-3beta,20-diol (7)。在评估七种三萜类化合物 (1-7) 对 Raji 细胞中 12-O-tetradecanoviphorbol-13-acetate (TPA) 诱导 Epstein-Barr 病毒早期抗原 (EBV-EA) 的抑制作用后，三种化合物 (5-7) 显示出对 EBV-EA 诱导的有效抑制作用（IC50 值为 277-420 摩尔比/32 pmol TPA）。
The structure of a triterpenoid isolated from the nonsaponifiable lipid (NSL) of the seed oil of the camellia (Camellia japonica L.; Theaccae) was established to be (20S)-3beta-hydroxy-25,26,27-trisnordammaran-24,20-olide (1; 3-epicabraleahydroxylactone) on the basis of spectroscopic and chemical methods. Six other triterpenoids isolated from the NSL were identified as 3-epicabraleadiol (2), ocotillol II (3), ocotillol I (4), dammarenediol II (5), (20R)-taraxastane-3beta,20-diol (6), and lupane-3beta,20-diol (7). Upon evaluation of the seven triterpenoids (1-7) with respect to their inhibitory effects on the induction of Epstein-Barr virus early antigen (EBV-EA) by 12-O-tetradecanoviphorbol-13-acetate (TPA) in Raji cells, three compounds (5-7) showed potent inhibitory effects against EBV-EA induction (IC50 values of 277-420 mol ratio/32 pmol TPA).