Detection of prolactin receptor (PRL-R) mRNA in the rat hypothalamus and pituitary gland.
Detection of prolactin receptor (PRL-R) mRNA in the rat hypothalamus and pituitary gland.
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DOI：
10.1210/endo.130.3.1537321
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发表时间：
1992-03
期刊：
影响因子：
4.8
通讯作者：
S. Chiu;R. D. Koos;P. M. Wise
中科院分区：
文献类型：
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作者：
S. Chiu;R. D. Koos;P. M. Wise
催乳素受体(PRL-R)mRNAs存在于多种组织中，其中包括肝脏、睾丸、前列腺、卵巢、乳腺、肾上腺和肾脏。催乳素也在下丘脑和脑下垂体的水平上起作用，反馈和调节自身的分泌和其他脑下垂体激素的分泌。因此，我们假设PRL-R mRNA也存在于这些靶组织中。提取大鼠下丘脑前、内侧基底核、垂体前、后叶、大脑皮层、骨骼肌和肝脏的总RNA。用小鼠MLV逆转录酶和随机或寡聚(DT)聚合酶逆转录总RNA后，进行聚合酶链式反应(PCR)。聚合酶链式反应产物经溴化乙锭染色分析。利用细胞外结合结构域编码区两侧的引物，我们检测到PRL-R在下丘脑前部和内侧、垂体腺前部和后部以及肝脏中的表达，但在大脑皮层和骨骼肌中检测不到。此外，当我们使用区分长形式和短形式的PRL-R mRNA的引物时，在同一组织中都可以检测到这两种形式的PRL-R mRNA。我们的数据提示，PRL可能通过在外周组织中介导PRL作用的相同的短和/或长的PRL-R mRNA在下丘脑和脑垂体水平上进行反馈。
Prolactin receptor (PRL-R) mRNAs exist in several tissues where prolactin is known to act including the liver, testes, prostate, ovary, mammary gland, adrenal gland and kidney. PRL also acts at the level of the hypothalamus and pituitary gland to feed back and regulate its own secretion and the secretion of other anterior pituitary hormones. Therefore, we hypothesized that PRL-R mRNA would exist in these target tissues as well. Total RNA was extracted from rat anterior and medial basal hypothalamus, anterior and posterior pituitary gland, cerebral cortex, skeletal muscle and liver. After reverse transcribing total RNA with Murine-MLV reverse transcriptase and random or oligo(dT) pmers, the polymerase chain reaction (PCR) was performed. PCR products were then analyzed by ethidium bromide staining. Using primers that flanked the coding region for the extracellular binding domain we detected PRL-R mRNA in the anterior and medial basal hypothalamus, anterior and posterior pituitary gland, as well as in the liver, but not in the cerebral cortex or skeletal muscle. In addition, when we used primers that distinguish the long and short forms of the PRL-R mRNA, both forms of the PRL-R mRNA were detectable in the same tissues. Our data suggest that PRL may feed back at the level of the hypothalamus and pituitary gland through the same short and/or long PRL-R mRNA that mediate PRL action in the peripheral tissues.