MicroRNA-432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes
MicroRNA-432 contributes to dopamine cocktail and retinoic acid induced differentiation of human neuroblastoma cells by targeting NESTIN and RCOR1 genes
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DOI：
10.1016/j.febslet.2014.03.015
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发表时间：
2014-05-02
期刊：
影响因子：
3.5
通讯作者：
Bhattacharyya, Nitai Pada
中科院分区：
文献类型：
--
作者：
Das, Eashita;Bhattacharyya, Nitai Pada
microRNA（miRNA）调节蛋白质编码基因的表达，并且已经涉及多种细胞过程，包括神经元分化、细胞生长和死亡。为了鉴定miRNA在神经元分化中的作用，用多巴胺鸡尾酒和视黄酸处理SH-SY 5 Y和IMR-32细胞以诱导分化。对分化细胞中miRNAs的检测显示，许多miRNAs的表达发生了显著改变。在改变的miRNA中，人脑表达的miR-432诱导神经突突起，将细胞阻滞在G 0-G1期，降低细胞增殖，并可显著抑制NESTIN/内斯、RCOR 1/COREST和MEP 2。我们的研究结果表明，miR-432调节人神经母细胞瘤细胞的神经元分化。(C)2014年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
MicroRNA (miRNA) regulates expression of protein coding genes and has been implicated in diverse cellular processes including neuronal differentiation, cell growth and death. To identify the role of miRNA in neuronal differentiation, SH-SY5Y and IMR-32 cells were treated with dopamine cocktail and retinoic acid to induce differentiation. Detection of miRNAs in differentiated cells revealed that expression of many miRNAs was altered significantly. Among the altered miRNAs, human brain expressed miR-432 induced neurite projections, arrested cells in G0-G1, reduced cell proliferation and could significantly repress NESTIN/NES, RCOR1/COREST and MECP2. Our results reveal that miR-432 regulate neuronal differentiation of human neuroblastoma cells. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.