Mutations in the Drosophila gene extradenticle affect the way specific homeo domain proteins regulate segmental identity.
Mutations in the Drosophila gene extradenticle affect the way specific homeo domain proteins regulate segmental identity.
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DOI：
10.1101/gad.4.7.1209
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发表时间：
1990-07
影响因子：
10.5
通讯作者：
Mark Peifer;Eric Wieschaus
中科院分区：
文献类型：
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作者：
Mark Peifer;Eric Wieschaus
我们的特点是一个基因，extradenticle，这似乎与果蝇同源结构域蛋白的一个特定的子集，可能会影响他们的目标特异性相互作用。这种解释是基于检查的合子和母体的影响表型的齿外突变。在胚胎中，牙外体基因产物水平降低，发生前节和后节转化。果蝇的体节身份是由双胸复合体和双足复合体的产物介导的。这些同源结构域蛋白被认为在每个片段中特异性地调节不同的靶基因，从而导致不同的形态。extradenticle改变体节的身份，而不影响同源异型基因的表达模式。遗传测试表明，在牙体外突变体中，同源异型蛋白是功能性的，并在其正常的节段结构域中起作用，但节段身份被改变。甚至当同源异型蛋白质异位表达的异源启动子的控制下，牙外突变影响其后果。因此，在缺乏足够的牙体外产物的情况下，改变的节段形态是由特定同源结构域蛋白的功能后果的改变引起的，可能是通过改变其靶基因特异性。外齿也表现为母性。完全去除外牙体，母亲和合子，导致特定的改变分割，其中许多是由于未能维持的同源结构域蛋白的表达。
We characterized a gene, extradenticle, which seems to interact with a specific subset of Drosophila homeo domain proteins, possibly affecting their target specificity. This interpretation is based on an examination of the zygotic and maternal effect phenotypes of extradenticle mutations. In embryos with reduced levels of extradenticle gene product, anterior and posterior segmental transformations occur. Segmental identity in Drosophila is mediated by the products of the Antennapedia and bithorax complexes. These homeo domain proteins are thought to regulate different target genes specifically in each segment, resulting in different morphologies. extradenticle alters segmental identity without affecting the pattern of expression of homeotic genes. Genetic tests demonstrate that in extradenticle mutants, the homeotic proteins are functional and act in their normal segmental domains, yet segmental identities are altered. Even when homeotic proteins are ectopically expressed under the control of a heterologous promoter, extradenticle mutations affect their consequences. Thus, in the absence of sufficient extradenticle product, altered segmental morphology results from alteration of the functional consequences of specific homeo domain proteins, possibly through alterations in their target gene specificity. extradenticle is also expressed maternally. Complete removal of extradenticle, maternally and zygotically, leads to specific alterations in segmentation, many of which result from failure to maintain the expression of the homeo domain protein engrailed.