Disappearance of M(r) 25,000 protein, a new protein kinase substrate, in parallel with a kind of serpin during embryogenesis.
Disappearance of M(r) 25,000 protein, a new protein kinase substrate, in parallel with a kind of serpin during embryogenesis.
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M(r) 25,000 蛋白（一种新的蛋白激酶底物）在胚胎发生过程中与一种丝氨酸蛋白酶抑制剂同时消失。
DOI：
10.1080/10258140290027234
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
E. Hashimoto
中科院分区：
文献类型：
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作者：
J. Funami;N. Miyoshi;I. Sugimoto;E. Hashimoto
M(r) 25,000 蛋白 (pp25) 是最近在非洲爪蟾卵母细胞中检测到的一种蛋白激酶底物 [Hashimoto, E. et al. 2017]。 (1995) J.生物化学。 118, 453-460]，但该蛋白质的生理作用仍有待确定。为了阐明pp25的一些特征，制备了针对它的多克隆抗体，并使用各种组织和生物系统检查了该蛋白质的分布和数量变化。在蛋白质印迹分析中，当检查每个组织的热稳定性胞质部分时，仅在非洲爪蟾卵母细胞中检测到 pp25，而在其他青蛙组织中未检测到。尽管在黄体酮诱导的卵母细胞成熟过程中pp25的量显然没有变化，但pp25在Nieuwkoop/Faber阶段45-48左右的胚胎中随着pNiXa（一种丝氨酸蛋白酶抑制剂）的变化而消失。这些结果表明 pp25 在非洲爪蟾卵母细胞中发挥一些特定的作用，并且 pp25 的水平在胚胎发育过程中发生巨大变化。
M(r) 25,000 protein (pp25) is a protein kinase substrate detected recently in Xenopus laevis oocytes [Hashimoto, E. et al. (1995) J. Biochem. 118, 453-460], but the physiological role of this protein remains to be determined. In order to elucidate some characteristics of pp25, a polyclonal antibody was raised against it and the distribution and quantitative changes of this protein were examined using various tissues and biological systems. In Western blot analysis, pp25 was detected only in Xenopus oocytes and not in other frog tissues when the heat-stable cytosolic fraction from each tissue was examined. Although the amount of pp25 apparently did not change during oocyte maturation induced by progesterone, pp25 disappeared in embryos around Nieuwkoop/Faber stages 45-48 in parallel with the change of pNiXa (a kind of serpin). These results suggest that pp25 plays some specific role(s) in Xenopus oocytes and that the level of pp25 changes dramatically during embryonic development.