PROTOCADHERINS - A LARGE FAMILY OF CADHERIN-RELATED MOLECULES IN CENTRAL-NERVOUS-SYSTEM
PROTOCADHERINS - A LARGE FAMILY OF CADHERIN-RELATED MOLECULES IN CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1002/j.1460-2075.1993.tb05878.x
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发表时间：
1993-06-01
期刊：
影响因子：
11.4
通讯作者：
SUZUKI, S
中科院分区：
文献类型：
--
作者：
SANO, K;TANIHARA, H;SUZUKI, S
利用聚合酶链反应，我们分离了许多大鼠和人的cdna，其中推导出的氨基酸序列与钙粘蛋白的细胞外结构域序列高度同源。由这两种cdna定义的两种人类蛋白质的整个假定编码序列已经确定。这些分子的整体结构与经典的钙粘蛋白非常相似，但它们有一些独特的特征。细胞外结构域由六到七个与钙粘蛋白非常相似的亚结构域组成，但具有独特的性质。另一方面，细胞质结构域与经典钙粘蛋白没有明显的同源性。由于从爪蟾、果蝇和秀丽隐杆线虫中也获得了具有几乎相同特征的各种cdna，因此似乎在各种生物体中表达了相似的分子。我们暂时将这些蛋白命名为原钙粘蛋白。它们在大脑中高度表达，其表达似乎受到发育调节。这两种全长cdna在L细胞中表达的蛋白大小约为170或150 kDa，主要定位于细胞-细胞接触位点。此外，转染物还表现出细胞粘附活性。
Using the polymerase chain reaction, we have isolated numerous rat and human cDNAs of which the deduced amino acid sequences are highly homologous to the sequences of the extracellular domain of cadherins. The entire putative coding sequences for two human proteins defined by two of these cDNAs have been determined. The overall structure of these molecules is very similar to that of classic cadherins, but they have some unique features. The extracellular domains are composed of six or seven subdomains that are very similar to those of cadherins, but have characteristic properties. The cytoplasmic domains, on the other hand, have no significant homology with those of classic cadherins. Since various cDNAs with almost identical features were obtained also from Xenopus, Drosophila and Caenorhabditis elegans, it appears that similar molecules are expressed in a variety of organisms. We have tentatively named these proteins protocadherins. They are highly expressed in brain and their expression appears to be developmentally regulated. The proteins expressed from the two full-length cDNAs in L cells were approximately 170 or 150 kDa in size, and were localized mainly at cell-cell contact sites. Moreover, the transfectants showed cell adhesion activity.