Direct Electron Transfer of Dehydrogenases for Development of 3rd Generation Biosensors and Enzymatic Fuel Cells.
Direct Electron Transfer of Dehydrogenases for Development of 3rd Generation Biosensors and Enzymatic Fuel Cells.
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DOI：
10.3390/s18051319
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发表时间：
2018-04-24
期刊：
影响因子：
--
通讯作者：
Antiochia R
中科院分区：
文献类型：
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作者：
Bollella P;Gorton L;Antiochia R
近年来，基于脱氢酶的生物电催化被越来越多地开发，以开发新的生物电化学装置，如生物传感器和生物燃料电池，具有改进的性能。在某些情况下，脱氢酶能够与适当设计的电极表面直接交换电子，而不需要添加氧化还原介体，从而允许基于直接电子转移过程的生物电催化。在这篇综述中，我们简要地描述了脱氢酶的电子转移机制和生物电催化反应通过直接电子转移机制所需的一些特性。特别注意的是纤维二糖脱氢酶和果糖脱氢酶，这表明有效的直接电子转移反应。最新的生物传感器和生物燃料电池的基础上的两个酶的概述。将仔细研究各种策略，以制备改性电极，以提高设备的电子转移性能，所有的分析参数将被呈现，讨论和比较。
Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an added redox mediator, allowing bioelectrocatalysis based on a direct electron transfer process. In this review we briefly describe the electron transfer mechanism of dehydrogenase enzymes and some of the characteristics required for bioelectrocatalysis reactions via a direct electron transfer mechanism. Special attention is given to cellobiose dehydrogenase and fructose dehydrogenase, which showed efficient direct electron transfer reactions. An overview of the most recent biosensors and biofuel cells based on the two dehydrogenases will be presented. The various strategies to prepare modified electrodes in order to improve the electron transfer properties of the device will be carefully investigated and all analytical parameters will be presented, discussed and compared.