Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons
Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons
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DOI：
10.7554/elife.16088
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发表时间：
2016-09-26
期刊：
影响因子：
7.7
通讯作者：
Sharott, Andrew
中科院分区：
文献类型：
--
作者：
Garas, Farid N.;Shah, Rahul S.;Sharott, Andrew
皮质纹状体传入可以使表达小白蛋白（PV+）的中间神经元迅速减少纹状体投射神经元（SPN）的活动，从而形成纹状体输出。基底神经节回路动力学的方案通常认为纹状体PV+中间神经元是同质的，尽管在形式和功能上都存在相当大的异质性。我们表明，选择性共表达的另一种钙结合蛋白，促分泌素（Scgn），分离PV+在大鼠和灵长类纹状体的中间神经元成两个地形，生理和结构不同的细胞群。在大鼠中，这两个interneuron群体不同的放电率，模式和与皮质振荡在体内的关系。此外，确定PV+/Scgn+中间神经元的轴突优先靶向所谓的“直接通路”的SPN的胞体，而PV+/Scgn-中间神经元优先靶向“间接通路”的SPN。因此，这两个群体的中间神经元可以提供一个基板，通过它的纹状体输出途径可以快速和选择性地抑制随后介导的行为例程的表达。
Corticostriatal afferents can engage parvalbumin-expressing (PV+) interneurons to rapidly curtail the activity of striatal projection neurons (SPNs), thus shaping striatal output. Schemes of basal ganglia circuit dynamics generally consider striatal PV+ interneurons to be homogenous, despite considerable heterogeneity in both form and function. We demonstrate that the selective co-expression of another calcium-binding protein, secretagogin (Scgn), separates PV+ interneurons in rat and primate striatum into two topographically-, physiologically- and structurally distinct cell populations. In rats, these two interneuron populations differed in their firing rates, patterns and relationships with cortical oscillations in vivo. Moreover, the axons of identified PV+/Scgn+ interneurons preferentially targeted the somata of SPNs of the so-called 'direct pathway', whereas PV+/Scgn- interneurons preferentially targeted 'indirect pathway' SPNs. These two populations of interneurons could therefore provide a substrate through which either of the striatal output pathways can be rapidly and selectively inhibited to subsequently mediate the expression of behavioral routines.