The clinical significance of downregulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p in gastric cancer tumorigenesis
The clinical significance of downregulation of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p in gastric cancer tumorigenesis
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DOI：
10.3892/ijo.2014.2415
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发表时间：
2014-07-01
影响因子：
5.2
通讯作者：
Zhou, Yongning
中科院分区：
文献类型：
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作者：
Li, Hailong;Xie, Shoupin;Zhou, Yongning
胃癌中异常miRNA通常通过miRNA芯片从临床样本中筛选，但有报道表明，每种miRNA芯片筛选的结果差异较大，异常miRNA，特别是下调miRNA之间存在矛盾。有鉴于此，我们采用Human Cancer Pathway Finder miRNA PCR阵列，将7株胃癌细胞系AGS、SGC-7901、MKN-45、MKN-28、MGC-803、BCG-823和HGC-27与永生化正常胃癌细胞系GES-1在癌症通路相关miRNA表达谱进行比较，并进行qPCR验证，分析下调miRNA及其靶基因富集的KEGG通路和GO项的临床意义。在胃癌细胞系中，38个mirna表达上调，4个mirna表达下调。58例患者胃癌组织中mir-124-3p、mir-146a-5p、mir-155-5p和mir-335-5p 4种mirna的临床意义与邻近非肿瘤组织比较表明，mir-124-3p、mir-146a-5p、mir-155-5p和mir-335-5p低表达组分别比高表达组表现出更广泛的淋巴结转移、淋巴浸润、静脉浸润、高分期Borrmann型、淋巴浸润和分化差(P
Dysregulated miRNAs in gastric cancer are usually screened by miRNA microarray from clinical samples, however, reports have indicated that results of each miRNA microarray screening are considerably different, and dysregulated miRNAs, especially downregulated miRNAs were contradictory. In view of this, the Human Cancer Pathway Finder miRNA PCR array was applied to compare 7 gastric cancer cell lines AGS, SGC-7901, MKN-45, MKN-28, MGC-803, BCG-823, and HGC-27 with an immortalized normal gastric cell line, GES-1 in cancer pathway-related miRNA expression profile, followed by qPCR verification, the clinical significance of downregulated miRNAs and the Enriched KEGG pathways and GO terms of their target genes were analyzed. Thirty-eight miRNAs were upregulated, and four miRNAs were downregulated in gastric cancer cell lines. Clinical significance of 4 miRNAs including mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p in gastric cancer tissue compared with adjacent non-tumor tissues of 58 patients indicated that the low-expression group of mir-124-3p, mir-146a-5p, mir-155-5p and mir-335-5p showed more extensive lymph node metastasis, lymphatic invasion, venous invasion, high-stage Borrmann type, lymphatic invasion and poor differentiation than that of the high-expression groups, respectively (P