Diverse immunostaining patterns of mineralocorticoid receptor monoclonal antibodies.
Diverse immunostaining patterns of mineralocorticoid receptor monoclonal antibodies.
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DOI：
10.1016/j.steroids.2011.09.004
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发表时间：
2011-12-20
期刊：
影响因子：
2.7
通讯作者：
Gomez-Sanchez EP
中科院分区：
文献类型：
--
作者：
Gomez-Sanchez CE;Warden M;Gomez-Sanchez MT;Hou X;Gomez-Sanchez EP
盐皮质激素受体是一种广泛分布的配体激活的核转录因子，它被多种伴侣蛋白结合，这些伴侣蛋白根据其在细胞中的位置和是否与配体结合而改变其构象。我们描述了针对与MR的5-550个氨基酸相对应的大鼠重组蛋白产生的新的单抗的开发和特征，以产生识别特定构象中的受体的抗体。所研究的大多数单抗与我们用多肽同位素免疫产生的单抗相似，但有两个在适当的分子质量处检测到单一条带作为MR，并在神经元中具有明显的免疫染色特征。一个标记胞浆MR，另一个标记膜和胞浆，包括轴突。这些抗体将使研究各种生理和病理条件下MR的亚细胞定位成为可能。我们也证实，MR非常不稳定，需要特殊处理。
The mineralocorticoid receptor (MR) is a widely distributed ligand activated nuclear transcription factor that is bound by various chaperone proteins that alter its conformation depending upon its location in the cell and whether it is ligand-bound. We describe the development and characterization of new monoclonal antibodies produced against a rat recombinant protein corresponding to aminoacids 5-550 of the MR to produce antibodies that recognize the receptor in specific conformations. Most of the resulting monoclonal antibodies studied were similar to those we produced by immunization with peptide isotopes, however two detected a single band at the appropriate molecular mass as the MR and had distinct immunostaining characteristics in neurons. One labeled cytosolic MR, the other labeled membranes and cytosol, including axons. These antibodies will permit study of the subcellular localization of the MR under various physiological and pathological conditions. We have also confirmed that the MR is highly unstable and requires special handling.
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影响因子：
5.3
作者：
Galigniana, Mario D.;Erlejman, Alejandra G.;Piwien-Pilipuk, Graciela
通讯作者：
Piwien-Pilipuk, Graciela
DOI：
10.1161/hypertensionaha.110.161653
发表时间：
2011-03
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Gros R;Ding Q;Sklar LA;Prossnitz EE;Arterburn JB;Chorazyczewski J;Feldman RD
通讯作者：
Feldman RD
影响因子：
19.6
作者：
de Kloet, ER;van Acker, SABE;de Jong, W
通讯作者：
de Jong, W
DOI：
10.1073/pnas.80.19.6056
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KROZOWSKI, ZS;FUNDER, JW
通讯作者：
FUNDER, JW
影响因子：
4.8
作者：
Kalman, BA;Spencer, RL
通讯作者：
Spencer, RL