Disruption of Genetic Interaction Between Two Autosomal Regions and the X Chromosome Causes Reproductive Isolation Between Mouse Strains Derived From Different Subspecies
Disruption of Genetic Interaction Between Two Autosomal Regions and the X Chromosome Causes Reproductive Isolation Between Mouse Strains Derived From Different Subspecies
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DOI：
10.1534/genetics.106.062976
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发表时间：
2007-01
期刊：
影响因子：
3.3
通讯作者：
A. Oka;Toshihiro Aoto;Y. Totsuka;R. Takahashi;M. Ueda;Akihiko Mita;Noriko Sakurai-Yamatani;Hiromi Yamamoto;S. Kuriki;N. Takagi;K. Moriwaki;T. Shiroishi
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文献类型：
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作者：
A. Oka;Toshihiro Aoto;Y. Totsuka;R. Takahashi;M. Ueda;Akihiko Mita;Noriko Sakurai-Yamatani;Hiromi Yamamoto;S. Kuriki;N. Takagi;K. Moriwaki;T. Shiroishi
引发物种形成的生殖隔离可能是由属于遗传分化种群的生物体中多个基因座之间的不相容引起的。实验室 C57BL/6J 小鼠（主要源自家鼠）和源自日本野生小鼠（M. m. molossinus，遗传上与家鼠相似）的 MSM/Ms 品系是生殖分离的。它们的 F1 杂种具有可育性，但连续的杂交会导致不育。一个康体菌株 C57BL/6J-ChrXMSM 在 C57BL/6J 背景中携带 MSM/Ms 的 X 染色体，显示出雄性不育性，表明 MSM/Ms X 染色体与其他 C57BL/6J 染色体的遗传不相容。在这项研究中，我们利用精子形状异常进行了全基因组连锁分析和随后的QTL分析，精子形状异常是C57BL/6J-ChrXMSM雄性不育的主要原因。这些分析成功地检测到 1 号和 11 号染色体上与 X 染色体相互作用的重要 QTL。将 MSM/Ms 1 号和 11 号染色体引入 C57BL/6J-ChrXMSM 背景未能恢复精子头部形状，但确实部分恢复了生育能力。这一结果表明，这种遗传相互作用可能在两个菌株之间的生殖隔离中发挥着至关重要的作用。通过胞浆内单精子注射和无带体外受精对雄性不育的详细分析表明，C57BL/6J-ChrXMSM精子在穿透卵子透明带方面存在缺陷。
Reproductive isolation that initiates speciation is likely caused by incompatibility among multiple loci in organisms belonging to genetically diverging populations. Laboratory C57BL/6J mice, which predominantly originated from Mus musculus domesticus, and a MSM/Ms strain derived from Japanese wild mice (M. m. molossinus, genetically close to M. m. musculus) are reproductively isolated. Their F1 hybrids are fertile, but successive intercrosses result in sterility. A consomic strain, C57BL/6J-ChrXMSM, which carries the X chromosome of MSM/Ms in the C57BL/6J background, shows male sterility, suggesting a genetic incompatibility of the MSM/Ms X chromosome and other C57BL/6J chromosome(s). In this study, we conducted genomewide linkage analysis and subsequent QTL analysis using the sperm shape anomaly that is the major cause of the sterility of the C57BL/6J-ChrXMSM males. These analyses successfully detected significant QTL on chromosomes 1 and 11 that interact with the X chromosome. The introduction of MSM/Ms chromosomes 1 and 11 into the C57BL/6J-ChrXMSM background failed to restore the sperm-head shape, but did partially restore fertility. This result suggests that this genetic interaction may play a crucial role in the reproductive isolation between the two strains. A detailed analysis of the male sterility by intracytoplasmic sperm injection and zona-free in vitro fertilization demonstrated that the C57BL/6J-ChrXMSM spermatozoa have a defect in penetration through the zona pellucida of eggs.