Extract of Fructus Schisandrae chinensis Inhibits Neuroinflammation Mediator Production from Microglia via NF- B and MAPK Pathways
Extract of Fructus Schisandrae chinensis Inhibits Neuroinflammation Mediator Production from Microglia via NF- B and MAPK Pathways
复制标题
五味子提取物通过 NF-B 和 MAPK 途径抑制小胶质细胞产生神经炎症介质
DOI：
10.1007/s11655-018-3001-7
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发表时间：
2019-02-01
影响因子：
2.9
通讯作者：
Wang Xue-mei
中科院分区：
文献类型：
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作者：
Song Fang-jiao;Zeng Ke-wu;Wang Xue-mei
目的探讨五味子提取物（EFSC）对脂多糖（LPS）诱导的BV-2细胞的抗神经炎症作用及其可能的机制。方法从中国癌症研究所（ICR）小鼠胎儿胚胎（E17-18）皮质中分离原代皮质神经元。从新生 ICR 小鼠的额叶皮质中分离出原代小胶质细胞和星形胶质细胞。不同的细胞在特定的培养基中培养。将细胞分为5组：对照组、LPS组（仅用1μg/mL LPS处理）和EFSC组（分别用1μg/mL LPS和100、200或400mg/mL EFSC处理）。通过甲基噻唑基二苯基溴化四唑（MTT）比色法测试 EFSC 对细胞活力的影响。定量 EFSC 介导的对 LPS 诱导的促炎介质（如亚硝酸盐 (NO) 和白细胞介素 6 (IL-6)）产生的抑制作用，并通过 Hoechst 33258 细胞凋亡测定和结晶紫染色测定测试针对小胶质细胞介导的炎症损伤的神经元保护作用。通过蛋白质印迹分析或免疫荧光评估促炎标记蛋白的表达。 结果EFSC（200 和 400 mg/mL）降低 LPS 诱导的 BV-2 细胞中 NO、IL-6、诱导型一氧化氮合酶 (iNOS) 和环氧合酶 2 (COX-2) 的表达 (P
ObjectiveTo investigate the anti-neuroinflammation effect of extract of Fructus Schisandrae chinensis (EFSC) on lipopolysaccharide (LPS)-induced BV-2 cells and the possible involved mechanisms.MethodsPrimary cortical neurons were isolated from embryonic (E17-18) cortices of Institute of Cancer Research (ICR) mouse fetuses. Primary microglia and astroglia were isolated from the frontal cortices of newborn ICR mouse. Different cells were cultured in specific culture medium. Cells were divided into 5 groups: control group, LPS group (treated with 1 g/mL LPS only) and EFSC groups (treated with 1 g/mL LPS and 100, 200 or 400 mg/mL EFSC, respectively). The effect of EFSC on cells viability was tested by methylthiazolyldiphenyltetrazolium bromide (MTT) colorimetric assay. EFSC-mediated inhibition of LPS-induced production of pro-inflammatory mediators, such as nitrite oxide (NO) and interleukin-6 (IL-6) were quantified and neuron-protection effect against microglia-mediated inflammation injury was tested by hoechst 33258 apoptosis assay and crystal violet staining assay. The expression of pro-inflammatory marker proteins was evaluated by Western blot analysis or immunofluorescence.ResultsEFSC (200 and 400 mg/mL) reduced NO, IL-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expression in LPS-induced BV-2 cells (P