Application of NMR screening techniques for observing ligand binding with a protein receptor
Application of NMR screening techniques for observing ligand binding with a protein receptor
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DOI：
10.1002/mrc.1492
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发表时间：
2005-01
影响因子：
2
通讯作者：
S. Shimotakahara;K. Furihata;M. Tashiro
中科院分区：
文献类型：
--
作者：
S. Shimotakahara;K. Furihata;M. Tashiro
通过梯度光谱（WaterLOGSY）、饱和转移差和NOE泵NMR技术观察到的水配体用于鉴定配体与受体的结合。虽然这些实验最初是为了观察复合物中的配体而设计的，但它们的应用受到配体对靶分子的亲和力的限制。在此，通过结合双脉冲场梯度自旋回波和梯度定制激发WATERGATE序列，开发了改进的WaterLOGSY脉冲序列。使用核糖核酸酶T1-抑制剂系统研究了这些配体观察的NMR筛选技术的效率。版权所有© 2004年约翰威利父子有限公司。
Water ligand observed via gradient spectroscopy (WaterLOGSY), saturation transfer difference and NOE pumping NMR techniques were used to identify ligand binding with a receptor. Although these experiments were originally designed to observe ligands in complexes, their application is limited by the affinity of ligands towards target molecules. Here the improved WaterLOGSY pulse sequence was developed by incorporating the double pulsed field gradient spin‐echo and gradient‐tailored excitation WATERGATE sequences. The efficiency of these ligand‐observed NMR screening techniques was investigated using the ribonuclease T1‐inhibitor system. Copyright © 2004 John Wiley & Sons, Ltd.